【摘要】 目的探讨Th细胞及相关效应分子对儿童乙肝疫苗免疫效果的影响。方法对6月~4岁健康儿童抗-HBs阴性者,按0、1、6的免疫程序完成三剂次乙肝疫苗接种,免疫后1~3个月内复查抗-HBs,按随机原则抽取免疫后抗-HBs阳转和未阳转者各50名作为研究对象,比较两组间Th细胞百分含量及其效应因子IL-17、IL-22、γ-IFN表达是否存在差异。结果抗-HBs阳转组和抗-HBs未阳转组儿童Th1百分含量分别为(25.23±7.03)%、(18.18±6.98)%,Th17百分含量分别为(1.78±0.93)%、(2.58±1.32)%,Th22百分含量分别为(0.72±0.36)%,(1.35±0.55)%,IL-17分别为(47.45±28.59)pg/m L、(59.90±26.67)pg/m L,IL-22分别为(13.56±7.03)pg/m L、(18.28±9.23)pg/m L,γ-IFN分别为(10.87±5.43)pg/m L、(16.12±7.19)pg/m L,两组间差异均有统计学意义(P<0.05)。结论 Th1、Th17、Th22及其免疫效应因子IL-17、IL-22、γ-IFN可能参与疫苗免疫后的抗-HBs产生过程,进一步研究有助于更好的预测疫苗接种效果,指导疫苗接种。更多还原

【Abstract】 Objective To investigate effect of Th cells and effector molecules on the immunity of children immunized with hepatitis B vaccine.Methods According to 0,1,6 immune procedure,children aged from 6 months to 4 years were inoculated with hepatitis B vaccine.Anti-HBs antibodywere tested 1-3 months.Fifty anti-HBs antibody positive and negative children were randomly chosen after immunity and divided randomly into two groups and the Th cells percentage and cytokines such as EL-17,IL-22,γ-IFN in children of both groups were detected.Results In anti-HBs positive group and anti-HBs negative group,the percentages of Th1 cells were(25.23 ± 7.03)% and(18.18± 6.98)%,Th17 cells percentages were(1.78 ±0.93)%and(2.58 ±1.32)%,Th22 cells percentages were(0.72 ± 0.36)%,(1.35±0.55)%,IL-17 were(47.45±28.59) pg/mL,(59.90 ±26.67) pg/mL,IL-22 were(13.56 ±7.03) pg/mL,(18.28±9.23) pg/mL,γ-IFN were(10.87 ±5.43) pg/mL,(16.12±7.19) pg/mL,all exhibited statistically significant differences(P<0.05).Conclusion Thl,Thl7,Th22 cells and the effector molecules as IL- 17,IL-22,γ-IFN may participate in production of anti- HB anbody after immunization,further study is helpful for better forecasting effect of vaccination and guiding the vaccination.更多还原