【摘要】 目的探讨在肺癌葡萄糖代谢方面,新型葡萄糖代谢显像剂99 Tcm标记的葡萄糖氨荒酸盐配合物99 TcmO(DGDTC)2单光子发射计算机断层摄影术(single photon emission computed tomography,SPECT)显像替代18F-氟脱氧葡萄糖(18F-fluorodeoxyglucose,18F-FDG)正电子发射断层扫描(positron emission tomography,PET)显像的可行性。方法通过配体交换反应,制备99 TcmO(DGDTC)2配合物,HPLC分析法检测99 TcmO(DGDTC)2的放化纯。常规孵育Spca-1细胞备用。在分别加入99 TcmO(DGDTC)2和18F-FDG后,99 TcmO(DGDTC)2组分别于30min、1h、2h、3h和4h5个时间点测定Spca-1细胞内外放射性计数,18F-FDG组分别于10、20、30、60和120min 5个时间点测定Spca-1细胞内外的放射性计数,计算摄取率及细胞各个时间点的Cin/Cout值。结果成功制备了99 TcmO(DGDTC)2配合物。HPLC分析法测定99 TcmO(DGDTC)2放射性化学纯度≥95%。注入99 TcmO(DGDTC)2后30min时间点百分注射剂量率(injection dose rate,ID)为(0.26±0.02)%,1h为(0.60±0.05)%,2h为(0.68±0.04)%,3h为(0.85±0.05)%,4h为(1.0±0.13)%;Cin/Cout30min为1.0,1h为2.0,2h为2.9,3h为1.7,4h为0.6。随时间延长,Spca-1细胞摄取99 TcmO(DGDTC)2逐渐增加,2h时达到摄取最大比值,3和4h逐渐下降。注入18 F-FDG后10 min个时间点ID为(0.40±0.05)%,20min为(0.72±0.02)%,30min为(0.95±0.03)%,60min为(1.03±0.05)%,120min为(1.16±0.13)%;Cin/Cout10min为1.2,20 min为2.0,30 min为4.2,60 min为3.5,120 min为3.0。随时间延长,Spca-1细胞摄取18F-FDG逐渐增加,30min时达到摄取最大比值,2h时开始下降。结论 99 TcmO(DGDTC)2制备后放射稳定性较好,肺癌Spca-1细胞能较好地摄取99 TcmO(DGDTC)2,摄取规律与摄取18 F-FDG显像剂的规律相似。99 TcmO(DGDTC)2SPECT显像有望成为替代18F-FDG PET显像的葡萄糖代谢显像剂。更多还原

【Abstract】 OBJECTIVE To study the uptake of 99 Tcmlabeled glucose dithiocarbamate composition [99 TcmO(DGDTC)2]in pulmonary adenocarcinoma cell line Spca-1by analyzing the in vitro results of this new tracer and to observe the application value of Single-Photon Emission Computed Tomography with 99 TcmO(DGDTC)2on lung cancer.The probability of substitution for Positron emission tomography with 18F-fluorodeoxyglucose was investigated.METHODS The complex 99 TcmO(DGDTC)2was prepared by ligand exchange action.HPLC was adopted to measure its radionuclidic purity.Spca-1cells were incubated in conventional for use.There were two groups:in the first group,99 TcmO(DGDTC)2was added,and in the second group,18F-FDG was added into the cell plates.In the first group the uptake of the tracer was observed over 30 min,1h,2h,3hand 4hrespectively after phosphate buffered solution was added.In the second group,the uptake of the tracer was observed over 10 min,20min,30 min,60min,120 min respectively after phosphate buffered solution was added,then cells were collected and the radioactive uptake of the above two tracers in pulmonary adenocarcinoma cell line were measured by aγ-ray spectrometer respectively.RESULTS The 99 TcmO(DGDTC)2complex:the 99 TcmO(DGDTC)2complexes were successfully prepared and the radionuclidic purity of 99 TcmO(DGDTC)2was ≥95%by method of HPLC.In vitro experiment as shown in the cells,the radio uptake of 99 TcmO(DGDTC)2increased gradually over time,the injection dose rates were(0.26±0.02)%,(0.60±0.05)%,(0.68±0.04)%,(0.85±0.05)% and(1.0±0.13)%for 30 min,1h,2h,3hand 4hrespectively.The uptake of the tracer by Spca-1cells reached the maximum in two hour(Cin/Cont was 2.9),and then gradually decreased after 3or 4hours.Compared with the 99 TcmO(DGDTC)2group,the 18F-FDG group the uptake of the tracer also showed a gradual increase over time,the uptake of the tracer by Spca-1cells reached the maximum at 30 min post-administration(Cin/Cont was 4.2),and then decreased gradually after 2hours.CONCLUSIONS The new tracer 99 Tcmlabeled glucose dithiocarbamate composition [99 TcmO(DGDTC)2]can be prepared with good radio stability.The experimental study in vitro showed the high uptake of 99 TcmO(DGDTC)2in pulmonary adenocarcinoma cell line Spca-1.It has the similar regularity of the uptake of the tracer with the 18F-FDG’s.This study implicates the potential application value of 99 TcmO(DGDTC)2in the imaging diagnosis of lung adenocarcinoma.更多还原