【摘要】 目的明确外阴阴道念珠菌病(VVC)患者分离的念珠菌种类及对抗真菌药物的敏感性。方法选取2003年4月—2012年9月就诊于北京大学深圳医院妇科门诊的3 141例VVC患者为研究对象。采集念珠菌标本进行培养,采用API Candida鉴定分离菌株,采用Rosco纸片扩散法进行抗真菌药敏试验。结果共分离出3 182株念珠菌(从41例患者中分离出两种念珠菌)。其中白念珠菌分离最多,占85.0%(2 705株),其次依次为光滑念珠菌(338株)、近平滑念珠菌(49株)、热带念珠菌(31株)、酿酒酵母菌(23株)、克柔念珠菌(15株)、无名念珠菌(11株)、红酵母菌(6株)、鲁希特念珠菌(2株)及土生念珠菌(2株)。白念珠菌对氟康唑、伊曲康唑、咪康唑、克霉唑及制霉菌素的耐药率分别为1.1%(18/1 612)、2.2%(36/1 612)、4.2%(68/1 612)、0.9%(14/1 612)、0。结论白念珠菌是VVC主要致病菌,白念珠菌对抗真菌药物的耐药不常见。更多还原

【Abstract】 Objective To determine species and antifungal drug susceptibility of Candida from patients with vulvovaginal candidiasis( VVC). Methods We enrolled 3 141 patients with VVC who received treatment in gynecological clinic of Peking University Shenzhen Hospital from April,2003 to September,2012. Specimens of Candida were collected from enrolled patients,API Candida system was used to identify the species,and Rosco Disk Diffusion method was employed to test drug susceptibility. Results A total of 3 182 strains of two species’ candida were extracted from 41 patients. C. albicans was the predominant species( 2 705 strains,85. 0%) in VVC,followed by C. glabrata( 338 strains),C. parapsilosis( 49 strains),C. tropicalis( 31 strains), Saccharomyces cerevisiae( 23 strains), C. krusei( 15 strains), Candida famata( 11 strains),Rhodotorula sp.( 6 strains),C. lusitaniae( 2 strains) and Candida humicola( 2 strains). The drug resistance rate of C. albicans to fluconazole,itraconazole,miconazole,clotrimazole,and nystatin were 1. 1%( 18 /1 612),2. 2%( 36 /1 612),4. 2%( 68 /1 612),0. 9%( 14 /1 612) and 0. Conclusion Candida albicans is the predominant Candida species in patients with vulvovaginal candidiasis. Resistance of vaginal C. albicans to antifungal agents is infrequent.更多还原