【摘要】 目的:探讨趋化因子受体CCR7和血管生成拟态(VM)在肝门部胆管癌组织的表达及其与临床病理因素的关系。方法:免疫组织化学染色检测63例肝门部胆管癌标本和20例正常胆管组织CCR7的表达,PAS双重染色检测血管生成拟态的表达,将CCR7表达和VM形成与临床病理因素进行相关性分析。结果:63例肝门部胆管癌组织中,CCR7阳性率79.4%。正常胆管表达率10%;胆管癌组织25例存在血管生成拟态形成,正常组织中无Vm形成;与临床病例因素相关性分析,CCR7和VM在肝门部胆管癌中的表达与肿瘤的分化程度和淋巴结转移呈显著相关;单变量和多变量相关性分析CCR7、VM、分化程度和淋巴结转移与生存率有关,而CCR7是影响肝门部胆管癌生存率的独立性因素。结论:肝门部胆管癌存在CCR7和血管生成拟态的表达,两者呈正相关;CCR7和VM的表达与肝门部胆管癌的淋巴结转移和肿瘤分化程度有关;CCR7是影响肝门部胆管癌生存率的独立性因素。更多还原

【Abstract】 Objective: To investigate expression of chem okine receptor CCR7 and vasculogenic mimicry in hilar cholangiocarcinoma tissue and relationship with clinical pathological param eters.Methods: The expression of chemokine receptor CCR7 and vasculogenic mimicry was tested by immunohistochemical and dual staining method respectively in 63 hilar cholangiocarcinoma specimens and 20 normal bile duct specimens, and had correlation test with clinical pathological param eters. Results: CCR7 expression was positive in 79.4%(50/63)of hilar cholangiocarcinoma specimens, in only 10%(2/20)of normal bile duct specimens. There was 25 Vasculogenic mimicry form ation in 63 hilar cholangiocarcinoma specimens, and zero in normal bile duct specimens. Expression of CCR7 and V asculogenic mimicry were relevant to lymph node metastasis and differentiation(P<0.05). 4 significant factors(CCR7/VM /lymph node metastasis/differentuation)were related with survival rate by univariate analysis, whereas in multivariate analysis, only CCR7 retained the prognostic significance. Conclusions: CCR7 and vasculogenic mimicry expression were highly positive in hilar cholangiocarcinoma tissue, and they had positive correlation each other. Expression of CCR7and Vasculogenic mimicry were relevant to lymph node metastasis and differentiation, and CCR7 was independentfactor with survivalrate.更多还原