【摘要】 [目的]建立并优化了土壤中草铵膦的高效液相色谱/荧光检测器分析方法。[方法]土壤样品经水进行提取,提取液用氯甲酸-9-芴基甲酯(FMOC-Cl)进行衍生化,用乙酸乙酯对衍生化溶液进行萃取净化;Luna氨基色谱柱进行分离,水相直接进HPLC进行分析检测。[结果]土壤中添加0.10~10.0 mg/kg,添加回收率为79.9%~86.3%,相对标准偏差为1.81%~6.87%。[结论]方法准确、灵敏。用该检测方法对贵州、广西的农田土壤中草铵膦的降解进行了研究,结果表明草铵膦在2地的降解半衰期为1.4~1.5 d。更多还原

【Abstract】 [Aims] The high performance liquid chromatography with fluorescence detection(HPLC-FLD) method was developed and validated for determining glufosinate in soil. [Methods] The soil samples were extracted with water, and the extract was derivatized with 9-fluorenylmethyl chloroformate(FMOC-Cl). Afterward, the derivatization solution was extracted for three times with ethyl acetate and separated by Luna amino chromatographic column. Finally, the aqueous phase was directly subjected to HPLC. [Results] The average recoveries were 79.9-86.3% with the relative standard deviation of 1.81-6.87% at the spiked levels of 0.10-10.0 mg/kg. [Conclusions] The method is accurate and sensitive. The degradation dynamics of glufosinate in Guizhou and Guangxi soils were studied and the half-lives of glufosinate were 1.4-1.5 d.更多还原