5- enolpyruvyl- shikimate- 3- phosphate synthase(epsps) is a new gene of high resistance to glyphosate and Bt cry1 Ac M as an insect-resistent gene can expresse at high level in monocotyledon. In order to breed transgenic maize with stacking traits of insect-resistance and glyphosate-tolerance, the plant expression vector p CAMBIA1302-P35S::epsps-Tnos-Pubi::cry1Ac M-Tnos was constructed and then introduced into maize(Zea mays) inbred lines by Agrobacterium mediated shoot apical meristem transformation. Afte...
【基金】
国家转基因生物新品种培育重大专项课题(No.2011ZX08003)
【更新日期】
2015-01-30
【分类号】
S513
【正文快照】
虫害是制约我国玉米高产的重要因素,其中以司从根癌农杆菌CP4(Agrobacterium sp.CP4)中克隆亚洲玉米螟分布最广,危害最重(王振营等,出的CP4-epsps基因(Barry et al.,1992),该基因已被2000)。杂草不仅与玉米植株争夺良好的生长环广泛应用于转基因大豆、玉米、棉花和油菜等植物境