【摘要】 本研究用改良碱煮法提取玉米叶片基因组DNA,探索了DNA模板量、引物量、PCR保护剂对PCR反应的影响。结果表明,当DNA模板原液稀释5倍,质量浓度为27.3 ng·μL-1,且反应体系中加入保护剂PVP-40和BSA时可有效提高PCR效率,结合双重PCR的方法实现了快速高效的交换单株筛选。更多还原

【Abstract】 In this study,a modified alkaline lysis method was used for the genomic DNA extraction from maize leaf,and then the amount of DNA template was studied and the PCR protectants,PVP-40 and BAS,were used. The results show that it was efficient for PCR reaction when DNA template was27. 3 ng·μL- 1and the protectants were used. In addition,the double PCR with the two sets of primers can be used to screen the recombinants with high efficiency.更多还原