【摘要】 基于纳米孔技术的新兴单分子传感技术已开发用于DNA测序,使得测序技术读取数据更快、测序成本降低、测序样品简化、无需标记.纳米孔孔内集成横向纳米电极可实现二维双通道同时检测易位信号的变化,从而提高纳米孔测序的精确度.目前纳米电极的制备方法主要包括:机械可控劈裂结法、电子束光刻法、电子束诱导沉积法、聚焦离子束刻蚀法、透射电镜刻蚀法等.分析纳米电极不同制备方法、应用范围、表征及其优缺点,可为后续纳米孔技术应用到DNA测序平台提供高效、稳定的选择.更多还原

【Abstract】 Single molecule sensing techniques based on nanopore,as an emerging technique,has been applied in DNA sequencing with faster reading,lower cost,simpler sampling and label-free advantages.Dynamic changes of translocation events can be recognized by nanopore with embedded transverse nanoelectrodes. Then two-dimensional signal can be acquired,which improves the accuracy of the DNA sequencing. The fabrication methods of the nanoelectrode mainly include mechanically controllable break junction,electron beam lithography,electron beam induced deposition,focused ion beam etching and transmission electron microscope etching. We reported on the fabrication,application and characterization of nanopore integrated with nanoelectrodes,and analyzed the advantages and disadvantages of these methods. This is an important platform for nanopore applied to ultrafast DNA sequencing by nanoelectrodes.更多还原