【摘要】 目的:探讨Toll样受体TLR2、4基因多态性与尖锐湿疣易感性及复发的相关性。方法:采用Snapshot方法对140例尖锐湿疣患者及105例HPV检测阴性的对照组外周血TLR2 597(T/C)、1350(T/C)、15607(A/G)、2408(G/A)和TLR4 896(A/G)、1196(C/T)进行基因多态性检测,并对治疗后6个月复发及无复发的尖锐湿疣患者间进行比较分析。结果:尖锐湿疣组TLR2 597(T/C)中TT、TC及CC分别有72、48及20例,对照组分别有71,31及3例,尖锐湿疣组突变基因C基因频率31.43%,对照组17.62%,尖锐湿疣组显著高于对照组(χ2=12.04,P<0.01)。并且TLR2 597(T/C)的突变基因C基因频率在复发尖锐湿疣组为38.68%,无复发尖锐湿疣组为27.01%,复发尖锐湿疣组显著高于无复发尖锐湿疣组(χ2=4.16,P<0.05)。尖锐湿疣组TLR2 1350(T/C)中TT、TC及CC分别有74、49及17例,对照组分别有73、29及3例,尖锐湿疣组突变基因C基因频率为29.64%,对照组为16.67%,尖锐湿疣组显著高于对照组(χ2=11.05,P<0.01)。复发尖锐湿疣突变基因C频率为36.79%,无复发尖锐湿疣组为25.29%,复发尖锐湿疣组显著高于无复发尖锐湿疣组(χ2=4.18,P<0.05)。尖锐湿疣组TLR215607(A/G)AA,AG和GG分别为44、66例和30例,对照组分别为26、58和21例,尖锐湿疣组突变基因G与对照组相比无显著性差异(χ2=0.33,P>0.05)。TLR2 2508(G/A)及TLR4 896(A/G)、1196(C/T)在尖锐湿疣组及对照组均未检测到基因突变。连锁不平衡分析显示TLR2 597及1350紧密连锁(D’=1,r2=0.93)。结论:TLR2597(T/C)及1350(T/C)紧密连锁,不仅与尖锐湿疣的易感性相关,而且与尖锐湿疣治疗后的复发相关。更多还原

【Abstract】 Objective: To explore the correlation of the gene polymorphisms of Toll-like receptor 2( TLR2) and TLR4 with the susceptibility and recurrence of condyloma acuminatum( CA). Methods: Using Snapshot,we detected the gene polymorphisms of TLR2 597( T / C),1350( T / C),15607( A / G),and 2258( G / A) and TLR4 896( A / G) and 1196( C / T) in the peripheral blood of140 CA patients and 105 HPV-negative controls. We made comparisons between the CA patients and controls as well as between the cases of recurrent CA and those of non-recurrence at 6 months after treatment. Results: There were 72,48,and 20 cases of genotype TT,TC,and CC of TLR2 597( T / C),respectively,in the CA patients,as compared with 71,31,and 3 cases in the controls. The gene frequency of mutant C was 31. 43% in the patients,significantly higher than 17. 62% in the controls( χ2= 12. 04,P < 0. 01),and it was 38. 68% in the recurrent cases,remarkably higher than 27. 01% in the non-recurrent cases( χ2= 4. 16,P < 0. 05). There were 74,49,and 17 cases of genotype TT,TC,and CC of TLR2 1350( T / C),respectively,in the CA patients,as compared with 73,29,and 3 cases in the controls. The gene frequency of mutant C was 29. 64% in the patients,significantly higher than 16. 67% in the controls( χ2= 11. 05,P < 0. 01),and it was 36. 79% in the recurrent cases,markedly higher than 25. 29% in the non-recurrent cases( χ2= 4. 18,P < 0. 05). There were 44,66,and 30 cases of genotype AA,AG,and GG of TLR2 15607( A / G),respectively,in the CA patients,as compared with 26,58,and 21 cases in the controls. There was no significant difference in the gene frequencies of mutant G between the two groups( χ2= 0. 33,P > 0. 05). No mutant genes of TLR2 2508( G / A) or TLR4 896( A / G) and 1196( C /T) were detected in either the CA patients or the controls. Linkage disequilibrium analysis showed a tight linkage between TLR2 597( T/C) and 1350( T/C)( D’= 1,r2= 0. 93). Conclusion: TLR2 597( T / C) is tightly linked to 1350( T / C),which is correlated with both the susceptibility and the recurrence of condyloma acuminatum.更多还原