【摘要】 从大庆油田的土壤中筛选出了一株高效萘降解菌株DQ-1,初步鉴定为假单胞菌属（Pseudomonas sp.）.该菌接种到以3 g/L萘为唯一碳源的无机盐培养基中,30℃培养4 d,其降解率达到98.6%.研究了Cu²⁺、Cr⁶⁺、Fe³⁺、Zn²⁺和Pb²⁺对菌株DQ-1生长的抑制情况,生长抑制效应由强到弱的顺序为Zn²⁺>Cr⁶⁺>Cu²⁺>Fe³⁺>Pb²⁺,并利用直读电感耦合等离子体发射光谱仪（ICP-OES,美国VARIAN公司）测定培养体系中重金属离子的含量,结果表明重金属离子最终吸附在菌株细胞壁上.将DQ-1菌株接种到含有2.5 mg/g萘的灭菌土壤中,经过11d室温培养之后,萘的去除率为97.9%.更多还原

【Abstract】 A naphthalene-degrading strain DQ-1 was isolated from oil contaminated soil of Daqing Oil Field and identified as Pseudomonas sp., which could utilizing naphthalene as a sole carbon source for growth and degrading naphthalene up to 98.6% of existed in minimal medium（3 g/L） within 4 days. Study on the inhibition of Cu²⁺, Cr⁶⁺, Fe³⁺, Zn²⁺and Pb²⁺on the growth of strain DQ-1, the inhibition effect follows the order of Zn²⁺> Cr⁶⁺> Cu²⁺> Fe³⁺> Pb²⁺. With the test of ICP-OES, it proved that the biosorption as a possible mechanism of heavy metal resistance in DQ-1. After inoculating strain DQ-1 into the autoclaved soils containing 0.25% naphthalene and incubating it at room temperature for 11 days,97.9% naphthalene was removed.更多还原