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柠檬酸和EDTA对蓖麻生理特性和镉累积的影响

Effects of CA and EDTA on Physiological Characteristics and Cd Accumulation of Ricinus communis

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【作者】 张玉芬刘景辉杨彦明张雷李卫红

【Author】 ZHANG Yu-fen;LIU Jing-hui;YANG Yan-ming;ZHANG Lei;LI Wei-hong;Agricultural College of Inner Mongolia Agricultural University;Analysis Center of Inner Mongolia University of Nationalities;Rural Energy and Environmental Protection Station of Inner Mongolia;Tongliao Mental Health Center;

【机构】 内蒙古农业大学农学院内蒙古民族大学分析测试中心内蒙古农村生态能源环保站内蒙古通辽市精神卫生中心

【摘要】 采用盆栽试验研究了螯合剂EDTA和柠檬酸(CA)单施或配施对蓖麻(Ricinus communis)生理特性及修复Cd污染土壤能力的影响。结果表明:单施EDTA显著降低蓖麻地上生物量和叶绿素含量(P<0.05),显著影响蓖麻叶片抗氧化酶活性(P<0.05),10 mmol·kg-1EDTA甚至抑制SOD和POD活性;CA与EDTA联用可在一定程度上缓解EDTA对蓖麻的毒害作用,CA可缓解1和5 mmol·kg-1EDTA导致的蓖麻地上生物量和叶绿素含量降低,缓解1 mmol·kg-1EDTA导致的MDA含量升高;单施5 mmol·kg-1EDTA蓖麻对Cd的富集和转移能力最高,但单株Cd富集量未达最大值,而2 mmol·kg-1CA与5 mmol·kg-1EDTA配施却使蓖麻单株总Cd富集量和提取率达到最高,分别是对照的2.98和3.00倍。因此,CA与EDTA联用在强化蓖麻修复Cd污染土壤中具有很好的应用潜力。

【Abstract】 A pot experiment was conducted to study effects of citric acid( CA) and EDTA on physiological characteristics and Cd accumulation of R. communis. Results showed that the application of EDTA alone significantly reduced the biomass of shoots and the content of chlorophyll of R. communis,evidently affected the activity of antioxidant enzyme in leaves of the plant( P < 0. 05),and even inhibited the activities of SOD and POD,when the application rate reached 10 mmol·kg-1. The application of EDTA and CA together reduced the toxicity of EDTA to R. communis to a certain extent,the effect of EDTA,at 1 or 5 mmol·kg-1,lowering the biomass of shoots and the content of chlorophyll of R. communis and the effect of EDTA,at 1 mmol·kg-1,increasing the content of MDA in the plant. The application of EDTA alone,at 5mmol·kg-1,brought Cd accumulation and transfer capacity of the plant to the peak. However,once 2 mmol·kg-1CA was applied in combination,the effect of EDTA reached a new peak,3 times as high as in CK. Therefore,combined application of EDTA with CA should be a practice of great potential in enhancing the effect of R. communis remedying Cd polluted soils.

【关键词】 蓖麻柠檬酸EDTA生物量
【Key words】 Ricinus communisCAEDTAbiomassCd
【基金】 农业部农业环境保护项目(20140420);国家自然科学基金(31360321);内蒙古自然科学基金(2015MS0340)
  • 【文献出处】 生态与农村环境学报 ,Journal of Ecology and Rural Environment , 编辑部邮箱 ,2015年05期
  • 【分类号】X173;S565.6
  • 【被引频次】29
  • 【下载频次】388
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