【摘要】 接骨木镰刀菌是马铃薯干腐病的主要致病菌,给黑龙江省马铃薯造成了一定的经济损失。采用马铃薯干腐病引物LDJ1对马铃薯干腐病、马铃薯晚疫病、马铃薯早疫病、马铃薯黑痣病的致病菌的ITS序列进行测定,只有马铃薯干腐病菌扩增出560 bp特异性片段。再分别对接骨木镰刀菌、燕麦镰刀菌和茄病镰孢变种的ITS序列进行BLAST比对分析,针对接骨木镰刀菌设计了特异引物L2,扩增特异性产物为278 bp,该引物所建立的PCR检测体系可检测DNA含量在50 pg/μL以上的目的基因。更多还原

【Abstract】 F. sambucinum is the main pathogenic bacterium bringing about potato dry rot and causes economic losses to some extent in Heilongjiang Province. Tests were made to the ITS sequence of Fusarium spp., Phytophthora infestans,Alternaria solani and Rhizoctonia solani using the primer LDJ1, and 560 bp specific fragment was amplified only from Fusarium spp.. Comparative analysis of BLAST was conducted on ITS sequence of F. sambucinum, F. avenaceum, and F. solani var.coeruleum, and the specific primer L2 was designed. The target band amplified was 278 bp. The sensitivity of PCR detection system based on this primer was 50 pg/μL for the target gene.更多还原