【摘要】 目的探讨NLRP7、P57和Ki-67免疫组织化学染色在早期葡萄胎的表达情况及应用价值。方法根据葡萄胎病理学诊断标准结合P57的表达,将确诊的葡萄胎分为部分性(PHM)和完全性葡萄胎(CHM),利用免疫组织化学染色检测妊娠早期的部分性葡萄胎(n=22)和完全性葡萄胎(n=24)石蜡组织切片中NLRP7和Ki-67蛋白的表达。结果 NLRP7蛋白在部分性葡萄胎和完全性葡萄胎的表达率分别为54.55%(12/22)和50%(12/24),差异无统计学意义(P>0.05),但CHM组NLRP7蛋白的表达强度组内比较有统计学差异,且强阳性表达组和部分性葡萄胎组相比,有显著统计学差异(P<0.01)。Ki-67在部分性和完全性葡萄胎组表达率分别为59.09%(13/22)和79.17%(19/24),有统计学差异(P<0.05)。结论 NLRP7蛋白单项检测不可直接用于鉴别部分性和完全性葡萄胎,需进一步检测基因突变,但联合P57和Ki-67蛋白可为复发性葡萄胎的预防及监测提供指导意义。更多还原

【Abstract】 Objective To investigate the expression of NLRP7,P57 and Ki-67 in hydatidiform mole. Methods Immunohistochemistry was used to detect the expression of NLRP7,P57 and Ki-67 in paraffin-embedded tissue sections of 22 partial hydatidiformmole( PHM) cases and 24 complete hydatidiformmole( CHM) cases in early trimester of pregnancy. Results Positive staining rates of P57 were 86. 36%( 19 /22) in PHM and 0. 0%( 0 /24) in CHM. There was no statistical difference in the expressions of NLRP7 between PHM and CHM( P > 0. 05),but the intensity of NLRP7 in PHM was weaker than that in CHM( P < 0. 01). Positive staining rate of Ki-67 was 59. 09%( 13 /22) in PHM and 79. 17%( 19 /24) in CHM respectively. There was no statistical difference in Ki-67 expression including distribution and intensity between PHM and CHM( P > 0. 05). Conclusion NLRP7 depending on the severity of its mutations was not suitable to distinguish PHM and CHM,however,co-detecting NLRP7,P57 and Ki-67 contributes to understanding and monitoring of recurrent hydatidiform mole.更多还原