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TGF-β1对rhBMP-2促进大鼠骨髓间充质干细胞增殖分化的影响
The Effects of TGF-β1 on the Growth and Proliferation of the Mesenchymal Stem Cells Induced by rhBMP-2
【摘要】 目的观察转化生长因子β1(TGF-β1)对重组骨形态蛋白2(rhBMP-2)促进大鼠骨髓间充质干细胞增殖分化的影响。方法分离培养骨髓间充质干细胞,然后加入不同浓度的rhBMP-2,用MTT法检测细胞增殖情况,通过ELISA法检测细胞碱性磷酸酶(ALP)活性评估其骨向分化能力。同时加入TGF-β1观察其对rhBMP-2协同促进能力。结果不同浓度rhBMP-2对骨髓间充质干细胞(MSCs)进行处理后检测出的吸光度值(D值)及ALP活性检测值均较阴性对照组明显升高(P<0.05),随着浓度增加,其促进作用有所提高。而TGF-β1+rhBMP-2组检测到的吸光度值及ALP活性升高更明显。结论 TGF-β1能够有效促进rhBMP-2对MSCs的增殖和成骨分化作用。
【Abstract】 Objective The research was to investigate the effect of transforming growth factor-β1(TGF-β1)on the growth and proliferation of the Mesenchymal stem cells after recombinant human bone morphogenetic protein-2(rhBMP-2) stimulating.Methods MSCs were isolated from bone marrow of male Sprague-Dawley(SD) rats and adhered to the culture plate in vitro.MSCs were divided into 3 groups as follows:(Group 1) control,(Group 2) rhBMP-2 in different concentrations,and(Group 3) TGF-β1 + rhBMP-2 in different concentration.MTT method was used to confirm the most effective concentration of rhBMP-2 on the proliferation of MSCs.ALP activity was evaluated by ELISA test.Results The number of MSCs and the ALP activity increased with rhBMP-2 in a concentration-dependent manner.A significantly increased number of MSCs was found at TGF-β1 + rhBMP-2 group(P <0.05).Conclusion The findings suggest that TGF-β1 + rhBMP-2 or rhBMP-2 alone promote the osteoblastic differentiation of MSCs.
- 【文献出处】 潍坊医学院学报 ,Acta Academiae Medicinae Weifang , 编辑部邮箱 ,2015年01期
- 【分类号】R329.28
- 【被引频次】4
- 【下载频次】2