【摘要】 为优化甘薯茎叶中黄酮类化合物的超声提取工艺,在单因素实验的基础上,选择乙醇浓度、溶剂倍量、超声提取时间为自变量,黄酮得率为响应值,采用Box-Behnken组合设计,运用Design-Export 8.05b软件分析各自变量及其交互作用对黄酮得率的影响,建立了黄酮得率的二次多项式回归方程的预测模型。确定甘薯茎叶中黄酮的最佳提取工艺条件为:乙醇浓度60.00%、溶剂倍量35.00、超声时间40.00min,在此条件下,黄酮得率为10.3002%。本研究原料价廉易得,提取方法高效可行,分析方法可信,可为甘薯茎叶的开发利用提供依据。更多还原

【Abstract】 In order to optimize the ultrasonic extraction of flavonoids from leaves and stems of Ipomoea batatas(L.)lam,on the base ofsingle-factor experiment,it took the following three parts as the independent variables:ethanol concentration,solvent amount of times andthe ultrasonic extraction time. It selected the flavonoid yield as response value. Then,it adopted the combination experiment design ofBox-Behnken centre,used Design Expert 8.05 b software to analyze the influence of the independent variables and its interaction on flavo-noids yield and established the predictable model of quadratic polynomial regression equation of flavonoids yield. The result ensured thebest extraction technology conditions are the following aspects:ethanol concentration is 60.00%,solvent amount of times is 35.00 and ul-trasonic extraction time is 40.00 min. In this condition,the actual flavonoids yield is 10.3002%. The extraction method has the advantageof time-saving,high efficiency and good reliablity of analysis method,which can provide the basis for the development and utilization ofthe leaves and stems of Ipomoea batatas(L.)lam.更多还原