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川芎嗪对脓毒症大鼠肝细胞线粒体体视学的影响

Effects of tetramethylpyrazine on stereology of hepatocyte mitochondria in septic rats

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【作者】 王锦权陶晓根张霖刘海华姚秀英张兴荣李坤Li Wei黄业华

【Author】 Wang Jinquan;Tao Xiao gen;Zhang Lin;Liu Haihua;Yao Xiu ying;Zhang Xingrong;Li Kun;Wei Li;Huang Yehua;ICU,Southside of Anhui Provincial Hospital Affiliated to Anhui Medical University;

【机构】 安徽医科大学附属省立医院安徽省立医院南区重症医学科UCL School of Pharmacy,University College London安徽省立医院肾内科

【摘要】 目的研究川芎嗪(TMP)对脓毒症大鼠肝细胞线粒体结构的保护作用及可能机制。方法实验大鼠被分成对照组、脓毒症组、治疗组和预防组;用盲肠结扎穿孔法制备大鼠脓毒症实验模型,在术后10 h,取肝脏标本用JEM-1230透射电子显微镜观察肝细胞的超微结构,选用Image-Pro Plus 6.0图像软件对线粒体进行测量;用Western blot技术检测肝细胞线粒体内膜水通道蛋白(AQP)8的表达。结果和对照组比较,其余3组单个肝细胞切面的线粒体总数和数密度均减少(P<0.01),但治疗组和预防组均多于脓毒症组(P<0.01);线粒体体积密度脓毒症组也较对照组低(P<0.01);线粒体内膜AQP8的相对表达量在脓毒症组、治疗组和预防组均较对照组明显减少(P<0.01);治疗组和预防组的AQP8相对表达量较脓毒症组增多(P<0.01)。结论在脓毒症状态下,TMP可保护肝细胞线粒体的结构,可能是通过增加线粒体内膜AQP8表达而起作用的。

【Abstract】 Objective To study the protective effects and plausible mechanisms of tetramethylpyrazine( TMP) on the structure of hepatocyte mitochondria in septic rats. Methods The rats were randomly divided into four groups: control group,septic group,therapeutic group and prevention group. The model of septic rat was established by the cecal ligation and puncture( CLP). The liver was taken out from septic rat 10 h after CLP,. The ultrastructure of hepatocyte were observed by the JEM-1230 transmission electron microscope. The hepatocyte mitochondria were measured by Image-Pro Plus 6. 0 analysis software. The expression of AQP8 was assayed by Western blot. Results Compared with the control group,the total number of mitochondria and numberical density were fewer in other 3 groups.The total number of mitochondria and numberical density of septic group were more than the therapeutic and prevention group( P < 0. 01),but the volume density of septic group was lower than the control group( P < 0. 01). Compared with the control group,the relative AQP8 protein expression was significantly reduced in the other 3 groups( P <0. 01). The AQP8 protein expression of the therapeutic and prevention group were higher than the septic group( P <0. 01). Conclusion TMP can protect stereology of hepatocyte mitochondria in septicrats by increasing the expression of AQP8 protein.

【基金】 安徽省国际合作项目(1403062022)
  • 【文献出处】 中国临床保健杂志 ,Chinese Journal of Clinical Healthcare , 编辑部邮箱 ,2015年04期
  • 【分类号】R459.7
  • 【被引频次】3
  • 【下载频次】85
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