【摘要】 目的通过观察华蟾素注射液(Cmobutacini)对人胰腺癌细胞株SWl990、BxPC3细胞周期的影响以探讨其抑制肿瘤细胞增殖的作用机理。方法采用CCK-8法检测法在不同的时间点(12 h、24 h、48 h、72 h和96 h)检测华蟾素注射液对人胰腺癌细胞株SW1990、BxPC3细胞增殖的影响;采用流式细胞术检测对细胞周期的影响。结果华蟾素注射液对人胰腺癌细胞株SW1990、BxPC3具有抑制细胞增殖的作用,并具有时间及剂量依赖性。其中对SW1990的半数抑制率浓度(IC50)为(8.92±1.98)mg/mL,对BxPC3的半数抑制率浓度(IC50)为(5.00±0.66)mg/mL。流式细胞术检测发现,华蟾素注射液可使人胰腺癌细胞株SW1990、BxPC3阻滞于的G2/M期。结论华蟾素注射液能通过诱导人胰腺癌细胞SW1990、BxPC3阻滞于G2/M期以抑制细胞增殖,为临床应用华蟾素注射液治疗胰腺癌提供实验依据。更多还原

【Abstract】 Objective To investigate the effects of Huachansu on proliferation and cell cycle distribution of pancreatic cancer cell lines SW1990 and BxPC3 and effective mechanism.Methods The anti-proliferation effects of Huachansu on human pancreatic cell lines SW1990 and BxPC3 were tested by CCK-8 assay for 12 hours,24 hours,48 hours,72 hours and 96 hours,respectively.The cell cycles were analyzed with flow cytometry.Results The data of in vitro study showed there were a concentration and time dependent anti-proliferation effects of Huachansu on pancreatic cancer cell lines.The half maximal inhibitory concentrations(IC50) for 72 hours anti-proliferation of SW1990 and BxPC3 were(8.92±1.98)mg/mL and(5.00±0.66)mg/mL,respectively.The cell cycle results showed that Huachansu could induce SW1990 and BxPC3 cells arrest at G2/M phase.Conclusions Huachansu is able to inhibit the growth of pancreatic cell lines and induce cancer cells arrested at G2/M phase.Collectively,these findings indicate that G2/M phase arrest contributes to the anti-proliferation effects in pancreatic cancer therapy.Therefore,Huachansu may be used as a therapeutic agent for the treatment of pancreatic cancer.更多还原