Connexin 43 co-locolizes and regulates the L type calcium channel current in atrial myocytes

【摘要】 Background Atrial fibrillation(AF) is the most common sustained cardiac arrhythmia without effective treatment. AF is associated with atrial conduction disturbances caused by electrical and / or structural remodel-ing. But the role of connexin(Cx) 43 in the regulation of L type calcium channel(LCC) remains unclear. We hypothesized that Cx 43 might co-localize and regulate the L type calcium channel current( ICa, L). Methods Real-time PCR and whole-cell patch clamp were used to detect the expression of LCC 1c subunit and the cur rent density of ICa, L, before and after Cx 43 knocking down respectively. The co-localization of Cx 43 with LCC was investigated by co-immunoprecipitation and confocal microscopy. Results Knocking down of Cx43 significantly inhibited the current density of ICa, Lthrough decreasing the gene expression of LCC α1c in cul tured atrium-derived myocytes(HL-1 cells). Cx43 co-localized with LCC α1c subunit in atrial myocytes.Conclusions Cx 43 regulates the ICa, Lin atrial myoctyes through LCC, representing a potential pathogenic mechanism in atrial arrhythmias.更多还原

【Abstract】 Background Atrial fibrillation(AF) is the most common sustained cardiac arrhythmia without effective treatment. AF is associated with atrial conduction disturbances caused by electrical and / or structural remodel-ing. But the role of connexin(Cx) 43 in the regulation of L type calcium channel(LCC) remains unclear. We hypothesized that Cx 43 might co-localize and regulate the L type calcium channel current( ICa, L). Methods Real-time PCR and whole-cell patch clamp were used to detect the expression of LCC 1c subunit and the cur rent density of ICa, L, before and after Cx 43 knocking down respectively. The co-localization of Cx 43 with LCC was investigated by co-immunoprecipitation and confocal microscopy. Results Knocking down of Cx43 significantly inhibited the current density of ICa, Lthrough decreasing the gene expression of LCC α1c in cul tured atrium-derived myocytes(HL-1 cells). Cx43 co-localized with LCC α1c subunit in atrial myocytes.Conclusions Cx 43 regulates the ICa, Lin atrial myoctyes through LCC, representing a potential pathogenic mechanism in atrial arrhythmias.更多还原