【摘要】 为了寻找一种快速、高效的体外分离、纯化鸡睾丸间质细胞的方法,本试验采用Ⅱ型胶原酶消化以及PercoⅡ分离液梯度离心,建立鸡睾丸间质细胞分离、纯化方法。结果表明,PercoⅡ分离液梯度离心后,纯化的间质细胞3β-羟基类固醇脱氢酶（3β-HSD）染色阳性率可达92.5%±1.7%,且在37℃、5%CO₂条件下培养的细胞生长良好。该提取方法效果良好,可作为研究禽类生殖调控的细胞模型。更多还原

【Abstract】 To find a high-efficient way to digest and purify rooster leydig cells.Methods:A multistep procedure including vascular perfusion,dissociation of type Ⅱ collagenase and PercoⅡgradient centrifugation was employed.Results:The purfied Leydig cell fraction after PercoⅡ density-gradient centrifugation contained 92.5%±1.7%well-preserved 3β-hydroxysteroid dehydrogenase（3β-HSD）,The better cultural condition was 37℃,5%CO₂.Conclusion:It suggests that the method is reliable and has somewhat applicant prospect.更多还原