【摘要】 目的探讨骨髓间充质干细胞(BMSC)移植对兔腹主动脉再狭窄和平滑肌细胞增殖的影响及机制。方法将60只新西兰大白兔随机分为对照组、损伤组、移植组,各20只。损伤组和移植组送入球囊扩张损伤腹主动脉,移植组以1×107/kg细胞数将荧光标记的BMSC注射到损伤血管局部。术后4周免疫组织化学染色腹主动脉平滑肌肌动蛋白(α-SM-actin)、增殖细胞核抗原(PCNA),计算PCNA增殖指数,血管形态计量分析。结果损伤组新生血管内膜层α-SM-actin表达明显强于对照组(P<0.05);与损伤组比较,移植组新生血管内膜α-SM-actin表达明显增强(P<0.05)。损伤组PCNA阳性细胞数较对照组明显增多(P<0.05);移植组PCNA阳性细胞数较损伤组明显减少(P<0.05)。损伤组腹主动脉内膜厚度和面积、中膜厚度和面积、血管狭窄程度等明显高于移植组和对照组(P<0.05);移植组上述指标与对照组比较,无统计学差异(P>0.05)。结论 BMSC血管局部移植可抑制兔腹主动脉血管平滑肌细胞的增殖,减轻动脉粥样硬化及狭窄程度。更多还原

【Abstract】 Objective To study the effect of BMSC transplantation on restenosis of abdominal aorta and proliferation of smooth muscle cells in rabbits after angioplasty and its mechanism.MethodsSixty New Zealand white rabbits were randomly divided into control group,injury group,transplantation group(20in each group).The abdovniual aorta was injured by balloon dilation in injury group and transplantation group,and fluorescence-labeled BMSC were injected into the local injured blood vessels of transplantation group at the speed of 1×107/kg cells.Smooth muscle actin and PCNA were stained with immunohistochemistry to calculate the PCNA proliferation index and analyze the morphology of blood vessels 4weeks after operation.Results The expression level ofα-SM-actin in new abdominal aorta intima was significantly higher in injury group than in control group(P<0.05)and in transplantation group than in injury group(P<0.05).The number of PCNA positive cells was significantly larger in injury group than in control group(P<0.05)and significantly smaller in transplantation group than in injury group(P<0.05).The abdominal aorta was significantly narrower,the abdominal aorta intima and media were significantly thicker,and their volumes were significantly larger in injury group than in transplantation group and control group(P<0.05).Although abdominal aorta stenosis was detected in transplantation group,no significant significance was found in its morphology between control group and transplantation group(P>0.05).Conclusion Local BMSC transplantation can inhibit proliferation of smooth muscle cells and alleviate atherosclerosis and stenosis of abdominal aorta.更多还原