【摘要】 目的探讨降钙素受体基因(calcitonin receptor,CTR)C1377T基因多态性与老年男性人群骨质疏松的相关性。方法前瞻性收集2014年8月至2015年8月在河北医科大学第三医院门诊就诊及住院患者中无亲缘关系且符合纳入及排除标准的老年男性220例,按照骨质疏松诊断标准分为骨质疏松症组(n=116)和非骨质疏松症组(n=104)。用聚合酶链反应限制性片段长度多态性技术检测其CTR基因多态性,并测量骨质疏松症组患者的跟骨定量超声参数:超声振幅衰减(broadband ultrasound attenuation,BUA)、超声声速(speed of sound,SOS)及刚度(systems technologies inc,STI)。通过比较两组人群中CTR基因型频率,并分析CTR基因型与跟骨定量超声参数之间的关系,探讨CTR基因多态性与老年男性骨质疏松症的相互关系。结果骨质疏松症组CTR基因型CC型占81.00%,CT型占19.00%;非骨质疏松症组C C型占7 2.1 0%,C T型占27.90%,两组比较差异无统计学意义(χ~2=2.450,P=0.118);骨质疏松症组C等位基因占90.52%,T等位基因占9.48%;非骨质疏松症组C等位基因占86.06%,T等位基因占13.94%,两组比较差异无统计学意义(χ~2=2.128,P=0.145)。但骨质疏松症患者CT基因型组的BUA、SOS和STI均明显高于CC基因型组,差异有统计学意义(P<0.05)。结论 CTR基因多态性不是影响骨质疏松发病的重要因素,但CTR基因型和定量超声参数所反映的骨密度、骨强度和骨的结构等指标均有一定相关性。更多还原

【Abstract】 Objective To investigate the relationship between the C1377 T polymorphisms of calcitonin receptor(CTR) gene and the incidence of osteoporosis in Chinese elderly male population. Methods A total of 220 elderly male outpatients who visited the clinic of the Third Hospital of Hebei Medical University between August 2014 and August 2015 were recruited, and divided into osteoporosis group(n=116) and non-osteoporosis group(n=104) according to the diagnostic criteria of osteoporosis. The genotype of CTR gene was examined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP). Broadband ultrasound attenuation(BUA), speed of sound(SOS) and systems technologies inc(STI) of osteoporosis patients were determined by UBIS3000 quantitative ultrasound(QUS). Results Genotypes CC and CT of CTR gene of osteoporosis group were 81.00% and 19.00%, the genotypes in normal group were 72.10% and 27.90% respectively, and there were no significant differences in the distribution of genotypes between these two groups(χ~2=2.450, P=0.118). Three quantitative ultrasound indexes in group of genotype CT were higher than those in genotype CC group. Conclusion CTR gene polymorphism may not be the important factors influencing the incidence of osteoporosis. But, CTR gene is connected with bone deity, bone strength and bone structure.更多还原