【摘要】 [目的]探讨不同剂量和时间2,4-二异氰酸甲苯酯(2,4-TDI)气体暴露对小鼠血清中特异性Ig G抗体水平的影响。[方法]将清洁级(SPF)BALB/c雌性小鼠64只,按照染毒时间7 d和14 d随机分为两部分,共8组,每组8只,每部分分别设对照组及低(0.036 mg/m3)、中(0.14 mg/m3)、高(0.36 mg/m3)浓度暴露组,采用TDI连续动式吸入染毒,每天染毒4 h,记录体重,于末次染毒24 h采血分离血清,采用酶联免疫法检测特异性Ig G含量。[结果]暴露7 d时,3个暴露组小鼠血清特异性Ig G抗体含量(光密度值)分别为(0.14±0.008 4),(0.14±0.006 4)及(0.14±0.007 6),与对照组(0.14±0.009 3)相比,差异无统计学意义(P>0.05);连续暴露14 d后小鼠血清中特异性Ig G抗体含量,各组间差异有统计学意义(F=11.41,P<0.05),进一步两两比较分析,中、高暴露组血清中特异性Ig G抗体含量分别为(0.17±0.020 0)和(0.18±0.021 0),均高于对照组(0.14±0.004 6)(P<0.05),高暴露组血清中特异性Ig G抗体含量高于低暴露组(0.15±0.012 0)(P<0.05);中暴露组的暴露14 d小鼠血清中特异性Ig G抗体含量为(0.17±0.020 0),高于暴露7 d(0.14±0.006 4)(P<0.05);高暴露组的暴露14 d小鼠血清中特异性Ig G抗体含量为(0.18±0.021 0),高于7 d(0.14±0.007 6)(P<0.05)。[结论]TDI气体连续暴露浓度及时间均影响小鼠血清中特异性的Ig G抗体水平的表达。更多还原

【Abstract】 [Objective] To examine the effects on serum specific Ig G antibody level in mice exposed to 2, 4-toluene diisocyanate(2, 4-TDI) at different doses and for different time. [Methods] Specific pathogen free(SPF) BALB/c mice(female, n=64) were randomly divided by the exposure time of 7 or 14 days into two parts with a total of eight groups containing eight rats per group. Each part included control group, low(0.036 mg/m3), middle(0.14 mg/m3), and high(0.36 mg/m3) dose groups. Rats in the latter three groups were continuously exposed to 2, 4-TDI vapor for 4 h/d. Weight was recorded and serum samples were collected 24 h following the final exposure. The levels of serum specific Ig G were detected by enzyme-linked immunosorbent assay. [Results] The average optical density level of specific Ig G antibody in each dose group on day 7(0.14±0.0084, 0.14±0.006 4, 0.14±0.007 6) had no significant difference compared with the control group(0.14±0.009 3)(P < 0.05). And the levels of specific Ig G antibody on day 14 were different among the groups(F=11.41, P < 0.05). The results of pair-wise comparison showed that the middle dose group(0.17±0.020 0) and the high dose group(0.18±0.021 0) presented higher levels of specific Ig G antibody than the control group(0.14±0.004 6)(P < 0.05), and the high dose group also presented higher level of specific Ig G antibody than the low dose group(0.15±0.012 0)(P < 0.05). The middle dose group on day 14(0.17±0.020 0) showed a higher level of specific Ig G antibody than on day 7(0.14±0.006 4)(P < 0.05). Similarly, the high dose group showed higher level of specific Ig G antibody on day 14(0.18±0.021 0) than on day 7(0.14±0.007 6)(P < 0.05). [Conclusion] Both concentration and time of TDI continuous exposure could influence the expression levels of serum specific Ig G antibody.更多还原