【摘要】 目的通过研究经导管动脉化疗栓塞(TACE)对肝癌细胞凋亡与自然杀伤(NK)细胞受体表达的变化,分析探讨TACE治疗中晚期原发性肝癌的临床价值。方法取经临床病理证实的原发性中晚期肝癌患者102例,运用采用TDT缺口末端标记TUNEL法和免疫组织化学SP法研究TACE治疗前后肝癌组织中肝癌细胞凋亡与NK细胞受体表达水平。结果 1、TACE治疗后凋亡指数(AI)升高,而增殖指数(PCNALI)降低(P<0.05),差异具有统计学意义;2、TACE治疗后NK细胞活化性受体NKG2D、NKp44、NKp30增多,颜色较深(P<0.05),差异具有统计学意义;活化性受体NKp46未见明显变化(P>0.05),差异无统计学意义;NK细胞抑制性受体CD158b、CD159a降低(P<0.05),差异具有统计学意义。结论 TACE治疗效果可能与诱导肝癌细胞凋亡及NK细胞活化性受体及抑制性受体变化相关联。更多还原

【Abstract】 Objective To explore the effect of transcatheter arterial chemoembolization(TACE) on the apotosis index( AI), the PCNA- Labeling index( PCNA- LI) and the NK cell receptor in hepatocellular carcinoma( HCC). Methods One hundred and three HCC specimens verified by histopathology were undergoing TACE. Terminal deox ynucleotidyl transferase-mediated d UTP nick end labeling(TUNEL) and immuno- histo chemistry method were used to observ e the apoptosis and PCNA;meantime eprole the by flow cytometry and immunohisto chemistry method. Results 1.HCCs treated with TACE had a signif-icantly higher AI and a low er PCNALI than those treated wit h surg ical resect ion alone(P<0.05). AI w as highly inversely related to PCNA- LI(P<0.05). 2. The expressions of NK-activating receptors NKG2 D, NKp44 and NKp30 were statistically higher in HCCs whichwere treated with TACE(P<0.05), the expression level of NKp46 showed no significant upregulation, while the expression levels of NK inhibitory receptors CD158 b and CD159 a were decreased(P<0.05). Conclusion HCCs treated with TACE could incr ease AI, decrease PCNA- LI,up-regulate the expressions of NKactivating receptors, and down-regulate the NK inhibitory receptors in the hepatic carcinoma.更多还原