【摘要】 目的 研究高脂饮食喂养诱导的胰岛素抵抗(IR)小鼠骨骼肌中磷脂酰肌醇3激酶(PI3K)表达及肌糖原含量的改变。方法 将20只雄性KM小鼠随机分为对照组(NC组,10只)和IR组(10只),NC组予常规饲料,IR组予高脂饲料。16周时IR造模成功,记录小鼠体质量、空腹血糖(FBG)和空腹胰岛素(FINS),并计算稳态模型胰岛素抵抗指数(HOMA-IR);Western blot法检测骨骼肌PI3K的表达,过碘酸雪夫(PAS)染色观察骨骼肌糖原含量的变化。结果 与NC组比较,IR组小鼠体质量、FBG、FINS及HOMA-IR增高(P均<0.05),IR组骨骼肌中PI3K的表达显著降低(P<0.05);PAS染色显示NC组骨骼肌细胞内紫红色着色广泛,着色颗粒较多,IR组着色浅,着色颗粒较少。结论 骨骼肌PI3K表达降低可能是导致肌肉IR产生的原因之一。更多还原

【Abstract】 Objective To study the changes in phosphatidylinositol 3-kinase(PI3K) expression and glycogen content in the skeletal muscle of mice with insulin resistance(IR) fed with high fat diet.Methods 20 male KM mice were randomly divided into normal control group(NC group,n=10) and IR group(n=10).The mice in NC group were fed with routine diet and the mice in IR group were fed with high-fat diet.At the 16 th week,IR models were tested successful.Record body weight,fasting blood glucose(FBG) and fasting insulin(FINS),then calculated Homeostasis Model Assessment for Insulin Resistance(HOMA-IR).The expression of PI3 K in the skeletal muscle were measured by Western blotting method and the muscle glycogen content were measured by Periodic Acid-Schiff Stain(PAS).Results Compared with NC group,the body weight,FBG,FINS and HOMA-IR of IR group were higher(respectively P < 0.05),the PI3 K in the skeletal muscle of IR group decreased significantly(P < 0.05).The skeletal muscle cells in NC control were colored extensively and more colored granules,while IR gruop were colored light and less colored granules.Conclusion The reduced expression of PI3 K in skeletal muscle may be one of the reasons leading to muscle IR.更多还原