【摘要】 目的:探讨环氧合酶2(COX-2)抑制剂塞来昔布对人腺样囊性癌细胞株(SACC-M)增殖和侵袭的抑制作用。方法:用CCK-8法检测不同浓度塞来昔布作用后SACC-M的增殖能力;用Western Blotting技术,比较塞来昔布作用24 h后的SACC-M中COX-2的表达;采用Transwell小室方法,检测塞来昔布作用24 h后的SACC-M的侵袭能力。结果:CCK-8法显示,塞来昔布对SACC-M细胞增殖有明显抑制作用,并表现出剂量依赖性。侵袭抑制实验表明,塞来昔布对SACC-M细胞的侵袭能力有抑制作用,与对照组SACC-M细胞相比较,有显著差异,其抑制作用具有浓度依赖性。Western Blotting实验得出,经过塞来昔布作用24 h后,SACC-M细胞中COX-2蛋白表达水平随塞来昔布浓度的升高依次降低。结论:塞来昔布对人腺样囊性癌细胞增殖与侵袭能力有明显抑制作用。更多还原

【Abstract】 Objective:To study the effects of cyclooxygenase-2(COX-2)selective inhibitor celecoxib on proliferation and invasion of human salivary adenoid cystic carcinoma cell line(SACC-M)in vitro. Method:The effect of celecoxib on the proliferation of SACC-M cell line was examined by the method of CCK8.The expression of COX-2 was measured by Western Blotting. The invasion of the SACC-M was determined by Transwell assay. Result:Celecoxib inhibited the proliferation of SACC-M by dose-dependent manner.After treated with celecoxib for 24 h,he invasion ability of SACC-M lower than the control group. The results of Western Blotting revealed that the 24 h treatment of celecoxib on SACC-M cell line inhibited the expression levels of COX-2. Conclusion:Celecoxib inhibits the proliferation and invasion of SACC-M cell line.更多还原