【摘要】 目的:通过观察经历不同时长实验性渐进性咬合紊乱后大鼠咬肌的超微结构及肌酸激酶(CK)和乳酸脱氢酶(LDH)含量变化的不同,以探讨渐进性咬合紊乱对大鼠咬肌的影响程度与时间之间的关系。方法:取8周龄SPF级雄性SD大鼠随机等分为2、4、6、8周4组,每个实验组各有一个空白对照组,每组8只,空白对照组不给予任何处理,实验组通过二次分牙操作,建立渐进性咬合紊乱模型,并分别于放入最后一个皮圈后2、4、6、8周处死,HE染色和透射电镜观察大鼠咬肌超微结构,同时采用酶联免疫法(ELISA)检测各大鼠咬肌中乳酸脱氢酶(LDH)及肌酸激酶(CK)的含量,以探讨咬合紊乱对咬肌能量代谢活动的影响,进一步增加对渐进性咬合紊乱与口颌系统活动关系的认识。结果:2、4、6、8周的渐进性咬合紊乱后,大鼠咬肌线粒体均出现损伤,且随时间的延长,损伤加重,CK及LDH的活性也逐渐增加(P<0.05)。结论:渐进性咬合紊乱对大鼠咬肌损伤与作用时间成正相关。更多还原

【Abstract】 Objective:To investigate the relationship between the effect of gradually induced occlusal disorders on the rat’s masseter muscle and disorded time by observing the changes of ultrastructure and the context of creatine kinase and lactate dehydrogenase in rat’s masseter muscle.Method:8 weeks’ male SD SPF rats were randomly divided into2,4,6,8 weeks groups,each of the experimental group had a blank control group with 8 rats in each group,the blank control group were not given any treatment.For the rats in experimental groups elastic rubber bands were inserted between the molar tooth to establish the disordered occlusion model.Experimental group and control group rats were sacrificed at the end of 2 th、4 th、6 th、8 th week respectively.The ultrastructure of masseter muscle in rats were observed by HE staining and using transmission electron microscope,at the same time using enzyme linked immunosorbent assay(ELISA)for detecte the activity of lactate dehydrogenase(LDH)and creatine kinase(CK)of masseter muscle in each rat,to explore the occlusal disorder effects on energy metabolism of masseter muscle.Result:After 2,4,6,8 weeks disordered occlusion,mitochondria in the masseter muscle of rats were damaged,and the longer the time disordered,the more damaged,and the more increased of CK and LDH(P < 0.05).Conclusion:the effect of disordered occlusion on masseter muscle of rat is time positively related.更多还原