【摘要】 目的探讨甘肃地区人群雌激素受体(ER)α-29位多态性与HBV感染相关原发性肝癌(PHC)发生的关系,从基因水平上探讨PHC的发病机制。方法采用聚合酶链反应-限制性片段长度多态性法检测106例HBV感染相关PHC患者、98例健康对照人群的ERα-29位多态性。用基因计数法计算检验人群的等位基因频率,进行Hardy-Weinberg遗传平衡定律检验。基因型及等位基因频率比较采用χ2检验。结果 HBV感染相关PHC患者ERα-29位多态性的TT基因型和T等位基因频率(31.1%,53.8%)明显高于对照组(11.2%,32.1%),差异有统计学意义(χ2值分别为3.449、3.840,P值均<0.05);CC基因型和C等位基因频率(23.6%,46.2%)明显低于后者(47.0%,67.9%),差异有统计学意义(χ2值分别为3.488、3.840,P值均<0.05);T等位基因发生HBV感染相关PHC的风险是C等位基因的2.46倍(OR=2.46,95%CI:1.64~3.69)。结论 ERα-29位T等位基因可增加HBV感染相关肝癌发病的风险。更多还原

【Abstract】 Objective To evaluate the relationship between estrogen receptor- α- 29( ERα- 29) gene polymorphisms and the development of HBV- related hepatocellular carcinoma( HCC) in Gansu Province,China,and to investigate the pathogenesis of HCC at the gene level. Methods Gene polymorphisms of ERα- 29 were analyzed in 106 HBV- related HCC patients and 98 healthy individuals as normal controls using the polymerase chain reaction- restriction fragment length polymorphism technique. Population allele frequencies were calculated using the gene counting method and then tested using the Hardy- Weinberg law of genetic equilibrium. Comparisons of genotype and allele frequencies between groups were performed using the χ2test. Results The frequencies of TT genotype and T allele of ERα- 29 gene in HBV- related HCC patients were significantly higher than those in the normal controls,i. e.,31. 1% and 53. 8% vs. 11. 2% and 32. 1%( χ2= 3. 449,P < 0. 05; χ2= 3. 840,P < 0. 05). In contrast,the frequencies of CC genotype and C allele of ERα- 29 gene in HBV- related HCC patients were significantly lower than those in the normal controls,i. e.,23. 6% and 46. 2% vs. 47. 0% and 67. 9%( χ2= 3. 488,P <0. 05; χ2= 3. 840,P < 0. 05). Compared with those carrying C allele,carriers of T allele had an increased risk( 2. 46- fold) of HBV- related HCC( OR = 2. 46,95% CI: 1. 64- 3. 69). Conclusion T allele of ERα- 29 gene can increase the risk of HBV- related HCC.更多还原