【摘要】 目的研究甘露糖结合凝集素基因(MBL2)多态性与肺结核易感性的关系。方法采用PCR限制性片段长度多态性(PCR-RFLP)对112例肺结核患者和120例健康对照DNA样本中MBL2-221位点和外显子区54号位点进行基因分型。结果肺结核病人和健康对照-221各基因位点之间有显著性差异(P<0.05)。在MBL2 6个单倍体型中,YA/YA基因型在肺结核病人和健康对照的比例分别为35.7%和49.2%,两者差异有统计学意义(P=0.038,OR值:0.57),XA/XA基因型则分别9.8%和1.7%(P=0.007,OR值:6.42),两者有显著性差异。肺结核病人-221位点和外显子区54号位点基因多态性除在不同年龄之间,初治与复治之间有显著性差异(P<0.05)外,其余临床特征与各位点基因多态性无关(P>0.05)。结论 YA/YA单倍体型可能是一种保护基因型,MBL基因多态性可能与肺结核易感性有关,并与肺结核病人复发等相关。更多还原

【Abstract】 Objective To study about the correlation between polymorphism of mannose binding lectin( MBL) genes and susceptibility to tuberculosis. Methods PCR-RFLP was used to detect DNA samples from 112 pulmonary tuberculosis inpatients and 120 health controls for the analysis of the MBL gene polymorphism( variants in-221 Y / X and exonl codons 54 A / B). Results There was significant difference in-221 gene sites between the study group and the control group( P < 0. 05). The frequency of YA / YA in the study group and the control group was35. 7% and 9. 2% respectively( P = 0. 038,OR value: 0. 57). The frequency of XA / XA was 9. 8% and 1. 7%( P= 0. 007,OR value: 6. 42). The significant differences of MBL gene polymorphism in-221 and codons 54 were only found between different age or initial treatment cases and re-treatment cases with tuberculosis( P < 0. 05). There was no significant difference between MBL gene polymorphism and other clinical characteristics( P > 0. 05). Conclusion The polymorphism of MBL gene may be associated with susceptibility to tuberculosis and recurrence of tuberculosis. The YA / YA may be a protected type against tuberculosis.更多还原