【摘要】 目的探讨肺泡表面活性蛋白D(SP-D)基因多态性与慢性阻塞性肺疾病易感性的关系。方法收集117例COPD患者(COPD组)和117名健康者(对照组)的外周血,提取全血细胞DNA,采用基因测序技术检测两组SP-D基因单核苷酸多态性位点(rs721917)的基因型分布频率。结果 COPD组中rs721917的CC、TC、TT频率分别为28.2%、56.4%、15.4%,与对照组41.0%、51.3%、7.70%相比(P=0.048);COPD组中T等位基因频率(43.6%)高于对照组(33.3%),T等位基因是COPD的易感基(OR=1.545,95%CI 1.062~2.249,P<0.05)。结论 SP-D(rs721917)位点基因多态性与COPD的易感性相关,T等位基因可能是COPD的易感基因。更多还原

【Abstract】 Objective To explore the association between genetic polymorphism of surfactant protein D( SPD) and susceptibility of chronic obstructive pulmonary disease( COPD). Methods The study enrolled 117 patients with COPD( the COPD group) and 117 healthy volunteers( the control group). Their peripheral blood and whole blood cell genomic DNA were detected. The genotype frequency of SP-D gene single nucleotide polymorphisms( rs721917) was tested by gene sequencing technologies. Results The frequencies of SP-D genotype( CC,TC,TT)were 28. 2%,56. 4% and 15. 4% respectively in the COPD group and 41. 0%,51. 3% and 7. 70% in the control group( χ2= 6. 063,P = 0. 048). The frequency of T allele gene was 43. 6% in the COPD group,which was higher than in the controls( 33. 3%). Conclusion SP-D( rs721917) genetic polymorphism is related to the development of COPD and T allele gene maybe a predisposing gene of COPD.更多还原