【摘要】 目的:研究黄酮类化合物对人工诱导的耐药金黄葡萄球菌的耐药基因mec A的mRNA表达水平的影响。方法:采用纸片法测定黄酮类化合物对耐药金黄葡萄球菌的逆转作用;利用RT-PCR法测定耐药基因mec A的相对表达量。结果:耐药金黄葡萄球菌分别被芦丁、姜黄素、木犀草素、槲皮素和芹菜素作用后,耐药基因mec A的相对表达量分别为0.55,0.79,0.76,0.92,和0.84。结论:黄酮类化合物能降低金黄葡萄球菌的耐药基因mec A的表达,能对抗金黄葡萄球菌的耐药性。更多还原

【Abstract】 Objective: To study the effects of flavonoids on the expression levels of mec A of Drug- resistant staphylococcus aureus. Methods: The reversal effects of drug- resistant staphylococcus aureus were determined by K- B paper methods. The expression levels of mec A were determined by RT- PCR method. Results: Rutin,curcumin,luteolin,quercetin and celery were acted on staphylococcus aureus,respectively which showed that the expression levels of mec A were 0. 55,0. 79,0. 76,0. 92 and 0. 84. Conclusion: Flavonoids can reduce the meca gene expression of drug- resistance Staphylococcus aureus,and it can compete against drug resistance of Staphylococcus aureus.更多还原