【摘要】 目的:探讨新型国产GBR胶原膜体内植入后,诱导早期膜下成骨的能力。方法:实验于2013年10月~2014年3月在沈阳军区总医院动物实验中心完成。选取小型巴马猪,于双侧下颌骨骨体处用牙科裂钻制备8mm×8mm全层骨缺损3个,分别应用实验胶原膜、Bio-gide@、无覆盖膜覆盖骨缺损。术后1个月处死动物,分别在处死前1、2周分别肌肉注射四环素溶液与二甲酚橙溶液。固定样本后,制备硬组织切片。分别在荧光显微镜及光学显微镜下(甲苯胺蓝、亚甲基蓝-酸性品红染色)观测膜的降解程度及膜下新骨生成能力,评价材料膜下骨形成量和骨成熟程度。结果:实验组胶原膜具备良好的屏障作用,膜下新生骨矿化程度良好;骨小梁排列整齐,但新生骨量少于对照组。结论:新型国产胶原膜在1个月时无明显降解,具备良好的膜下成骨能力,需进行实验组胶原膜的改性,以增加膜下成骨量。更多还原

【Abstract】 Objective:To evaluate a new synthetic degradable barrier membrane based on collagen for use in guided bone regeneration.Methods:The collagen membrane was embedded in Bama pigs mandibular bone and Bio-gide@membrane served as reference materials.In Bama pigs,three standardized 8mm×8mm defect was created in mandibular bone.Three groups(collagen,Bio-gide@,no membrane)were evaluated at 4weeks.New bone formation was demonstrated by histological analysis.Results:In the Bio-gide@and collagen groups,the defects were incompleted new formedbone filling.In contrast to uncovered mandibular defects,substantial bone healing was observed in defects covered with a barrier membrane.In the non membrane covered case,the formation of bone was unformed in4 weeks.There were new formed bone under the Bio-gide@ and collagen membranes.The amount of new bone formed under the Bio-gide@ membranes were more than the amount of bone formed under the collagen membranes.Conclusion:It can be concluded that the collagen membrane was well suited for use in guided bone regeneration.更多还原