【摘要】 microRNA(miRNA)是广泛存在于真核生物中的一种具有高度保守序列的非编码蛋白,长度为20到24个核苷酸,它参与细胞增殖、分化、凋亡等多种细胞活动的调控。其中miRNA448会降低脂肪细胞的分化,与甘油三酯的堆积和脂肪细胞的表达相关,参与肥胖的自我调控机制。而且miRNA-448在研究中被发现通过影响丙型肝炎病毒的复制,来影响机体被微生物感染后的免疫反应。同时在肝癌、乳腺癌、胶质瘤等肿瘤中的表达异常,成为一种新的生物标记物,并通过与多个靶基因SATB1,NF-κB和5-HT2B等的作用参与癌症分化、侵袭、转移。近来我们在口腔鳞状细胞癌(OSCC)中通过高通量深度测序技术构建miRNA和mRNA表达谱,发现miRNA-448在OSCC中对比癌旁组织增高10倍,为数百种检测的miRNA中增高最多,miRNA-448可能在OSCC中起着十分重要的作用,但是miRNA-448在OSCC中尚无任何研究报道,为了奠定miRNA-448在OSCC中作用的深化认识,因此在此对miRNA-448的研究进展进行综述。更多还原

【Abstract】 MicroRNA( miRNA) are highly conservative sequence of the coding protein widely existing in eukaryotic organism. The mature miRNA are 20 ~ 24 nucleotides,which can regulate a variety of cellular activities including proliferation,differentiation,and apoptosis. miRNA-448 can regulate adipocytes differentiation; it influences the accumulation of triglycerides and expression adipocytes,and also gets involved in the self-regulatory mechanisms of obesity. Moreover,it was found in a study that miRNA-448 could impact the immune response of the organism after microorganism infection through influencing the replication of hepatitis C virus. MiRNA-448 becomes a new biomarker due to its abnormal expression in the liver cancer、breast cancer、glioma and other tumors,and miRNA-448 is involved in cancer differentiation,invasion,transfer by the action of a plurality of target genes like STAB1、NF-κB and 5-HT2 B. Recently we built a expression profile of miRNA and mRNA through a high-throughput deep sequencing technology in oral squamous cell carcinoma( OSCC) and found that miRNA-448 increased by 10-fold compared to adjacent tissues and became the most elevated one in the hundreds of detected miRNA,so it may play an important role in OSCC. However,there is no research reporting miRNA-448 in OSCC yet. To enhance deep understanding of the role of miRNA-448 in OSCC,we are reviewing the progress of miRNA-448.更多还原