【摘要】 目的探讨Ⅰ型胶原和矿化Ⅰ型胶原合成的胶原膜作为GBR屏障膜在动物体内植入后,诱导早期膜下成骨的能力。方法实验于2013年10月—2014年3月在沈阳军区总医院动物实验中心完成。选取小型巴马猪双侧下颌骨,分别于下颌骨骨体处用牙科裂钻制备8 mm×8 mm全层骨缺损3个,分别应用实验胶原膜覆盖、Bio-gide@覆盖、无覆盖膜骨缺损区。术后1个月处死动物,在处死前1、2周分别肌肉注射四环素溶液与二甲酚橙溶液。固定样本后,制备硬组织切片。分别在荧光显微镜及光学显微镜下(甲苯胺蓝、亚甲基蓝-酸性品红染色)观测膜的降解程度及膜下新骨生成能力,评价材料膜下骨形成量和骨成熟程度。结果实验组胶原膜具备良好的屏障作用,膜下新生骨矿化程度良好;骨小梁排列整齐,但新生骨量少于Bio-gide@覆盖组;无覆盖膜骨缺损区新生骨组织骨小梁排列混乱,新生骨量少。结论新型胶原膜在1个月时体内无明显降解,具备良好的膜下成骨能力,下一步需进行实验组胶原膜的改性,以增加胶原膜膜下成骨量。更多还原

【Abstract】 Objective To evaluate a new collagen-based synthetic degradable barrier membrane for use in guided bone regeneration.Methods A new collagen / HA-collagen degradable barrier membrane was embedded in Bama pigs mandibular bone and Bio-gide@membrane served as reference materials. In Bama pigs,three standardized 8 mm × 8 mm defects were created in mandibular bone. Three groups( collagen / HA-collagen,Bio-gide@,no membrane) were evaluated at 4 weeks. New bone formation was demonstrated by histological analysis. Results In the experimental group,collagen membrane had good barrier function and mineralization degree of new bone under membrane was good. In contrast to uncovered mandibular defects,substantial bone healing was observed in defects covered with a barrier membrane. In the non membrane covered case,the formation of bone was unformed in 4 weeks. There was new formed bone under the Bio-gide@and collagen / HA-collagen membranes. The amount of new bone formed under the Bio-gide@membranes was more than that of bone formed under the collagen membranes. Conclusions It can be concluded that the collagen / HA-collagen membrane is well suited for use in guided bone regeneration.更多还原