【摘要】 目的探讨甲状腺转录因子-2转基因小鼠继发腭发育过程中,TTF-2 m RNA及蛋白表达的规律.方法于妊娠12 d、13 d、14 d、15 d分别断颈处死各3只孕鼠,无菌条件取出胚胎,解离腭突.应用RT-PCR技术和Western印记方法检测TTF-2在腭突中的表达.结果在甲状腺转录因子-2转基因小鼠胚胎的腭突形成、上抬和融合的发育过程中均有TTF-2的表达,但m RNA及蛋白表达水平变化无统计学意义(P>0.05),RT-PCR检测和Western印记结果一致.结论甲状腺转录因子-2转基因小鼠继发腭发育过程中,TTF-2在腭突中的持续高表达,这可能是甲状腺转录因子-2转基因小鼠出现腭裂的原因之一.更多还原

【Abstract】 Objective The study aimed to investigate the expression of TTF- 2 m RNA in palatal development of Titf- 2 transgenic mouse. Methods From the embryonic day 12(E12) to E15, three pregnant mice were decapitated to obtain the embryos in each day. Palatal shelves were separated from the Titf- 2 transgenic mouse embryos, and the TTF- 2 expression in palatal shelves was determined by RT- PCR and Western blot. Results TTF- 2 was highly expressed in the palatal shelves development of titf- 2 transgenic mouse involving palatal shelves growing, elevating and fusing, but there was no significant difference in the change of expression between m RNA and protein. The result of RT- PCR test was consistent with that of Western blot. Conclusion TTF- 2 is highly expressed in the palatal shelves from the embryonic day 12(E12) to E15 in Titf- 2 transgenic mice, and the overexpression of TTF- 2 during palatogenesis may contribute to the formation of cleft palate.更多还原