【摘要】 目的:观察HU-308药物缓释涂层对骨质疏松大鼠种植体周骨密度、类骨质形成和骨吸收的影响。方法:60只6月龄未交配雌性wistar大鼠,随机分为两组,A组15只,B组45只,A组切除双侧卵巢旁等量脂肪组织,B组通过双侧去卵巢手术建立骨质疏松模型。建模成功后B组45只大鼠随机均分为3组:C、P、H组,每组15只,术后3个月在所有大鼠双侧胫骨骭骺端按组别植入种植体,A、C组植入碱热处理种植体,P组植入肝素/壳聚糖涂层种植体,H组植入携带HU-308的肝素/壳聚糖涂层种植体。分别于种植术后4周、8周、12周随机处死各组大鼠5只,带种植体的胫骨制作不脱钙骨组织磨片,经甲苯胺蓝染色后,光镜观测并计算种植体螺纹内骨密度(BD)、类骨质周长百分数(%O.Pm)和骨吸收周长百分数(%Er.Pm)。结果:4周和8周时,A组和H组BD、%O.Pm显著高于C和P组(P<0.05),A组和H组%Er.Pm显著低于C和P组(P<0.05)。H组除BD低于A组外(P<0.05),其余指标与A组差异无统计学意义(P>0.05);12周时,A组BD显著高于C、P和H组(P<0.05),%O.Pm、%Er.Pm显著低于C、P和H组(P<0.05),而C、P和H组之间BD、%O.Pm、%Er.Pm差异无统计学意义(P>0.05)。结论:骨质疏松改变可使种植体周骨密度降低,抑制新骨形成,促进骨吸收;H U-308药物涂层在种植体植入早期可提高骨质疏松大鼠种植体周围骨密度,促进新骨形成,抑制骨吸收。更多还原

【Abstract】 Objective: To investigate the effects of HU-308 coating on bone density、osteoid formation and bone resorption around implants in osteoporotic rats. Methods: Sixty-four female wistar rats were randomly divided into two groups:control(A,15 rats) and ovariectomized(B,45 rats). Animals in group A received a sham operation while animals in group B underwent ovariectomy procedures. After setting up the osteoporosis models, 45 rats in group B were randomly divided into three groups: C, P and H. Different titanium implants were inserted in the proximal metaphyses of all rats’ bilateral tibias.Rats in group A and C received alkalization treatment implants. Rats in group P received heparin/chitosan coating implants.For group H, animals received heparin/ chitosan loaded HU-308 coating implants. At 4,8 and 12 weeks post-implantation,5 rats of each group were randomly sacrificed and the tibias with implants were harvested to prepare undecalcified grinding slices. After toluidine blue staining, the grinding slices were measured Bone density(BD), Percent osteoid perimeter(%O. Pm)and Percent eroded perimeter(%Er.Pm). Results: 4 and 8 weeks after implantation, BD and %O.Pm in group A and H were both significantly higher than group C and P(P<0.05), while %Er.Pm in group A and H was significantly lower than group C and P(P<0.05). BD in group H was significantly lower than group A(P<0.05), while %O.Pm and %Er.Pm in group H were similar to group A(P>0.05). 12 weeks after implantation, BD in group A was significantly higher than group C, P and H(P <0.05), and % O. Pm and % Er.Pm in group A were both significantly lower than group C, P and H(P <0.05)while the differences of BD, %O. Pm and % Er.Pm between group C, P and H were not significant(P>0.05). Conclusion:Osteoporosis could reduce bone density, inhibit new bone formation, and promote bone resorption around implants. HU-308 coating could increase bone density, promote new bone formation, and inhibit bone resorption around implants in osteoporotic rats at the early-middle stage of implantation.更多还原