【摘要】 目的了解儿童呼吸道肺炎支原体临床分离株对常用抗菌药物的敏感性。方法采用微量稀释法测定分离自呼吸道感染患儿的112株肺炎支原体对大环内酯类、四环素类和氟喹诺酮类药物的敏感性。对肺炎支原体核糖体23S rRNA全序列进行扩增及测序。结果 98株(87.5%)肺炎支原体临床分离株对红霉素和阿奇霉素耐药,耐药株均存在A2063G或A2064G的23S rRNA核苷酸点突变。四环素类和氟喹诺酮类对肺炎支原体仍具有良好的抗菌活性。结论呼吸道感染患儿肺炎支原体临床分离株对大环内酯类耐药率高,核糖体23S rRNA核苷酸点突变是导致其对红霉素和阿奇霉素耐药的原因。更多还原

【Abstract】 Objective To investigate the profile of antimicrobial susceptibility of the Mycoplasma pneumoniae(Mpn)strains isolated from pediatric patients with respiratory tract infection.Methods Antimicrobial susceptibility testing was conducted with a total of 112 Mpn clinical strains by broth microdilution method.Sequence analysis of full 23 S rRNA genes was performed for all Mpn strains.Results One hundred and twelve Mpn strains were isolated from January 2009 to March 2011.Of these clinical isolates,98(87.5%)were resistant to erythromycin and azithromycin.All macrolide-resistant Mpn strains harbored an A2063 G or A2064 G transition mutation in domain V of 23 S rRNA genes.Mpn isolates were still very susceptible to the tetracyclines and fluoroquinolones tested.Conclusions The Mpn strains from pediatric patients are highly resistant to macrolides.The mechanism of macrolide resistance may be associated withthe transition mutation on 23 S rRNA gene.更多还原