【摘要】 目的初步研究棘霉素(echinomycin)对KBV200细胞(耐长春新碱的口腔癌细胞)的作用及其作用机制。方法采用MTT法及流式细胞仪分别检测KBV200细胞的增殖和凋亡;用同位素掺入、Western blot、RT-PCR等方法分别检测鞘氨醇激酶、P-糖蛋白、缺氧诱导因子的表达。结果棘霉素可诱导KBV200细胞的凋亡,流式结果出现DNA亚二倍体凋亡峰;棘霉素对鞘氨醇激酶、P-糖蛋白、缺氧诱导因子的表达有不同程度的抑制作用。结论棘霉素对KBV200细胞有细胞毒作用,其作用机制与抑制鞘氨醇激酶、P-糖蛋白、缺氧诱导因子的表达有关。更多还原

【Abstract】 Objective To research the action and mechanism of echinomycin in KBV200 cell line.Methods The proliferation and cell cycle of KBV200 cells were determined by MTT assay and flow cytometry,respectively.TLC,Western-blot and RT-PCR were used to detect the expressions SPK,P-gp and HIF-1α on KBV200 cells,respectively.Results Echinoderm can induce apoptosis of KBV200 cells,and have different degrees of inhibition effect on expression of SPK,P-gp,HIF-1.Conclusion Echinomycin has better the cytotoxic effect on KBV200 cells,and the mechanism of action is related to inhibition of expression SPK,P-gp and HIF-1α.更多还原