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旋毛虫肌幼虫囊包标本HE染色制作方法

HE staining methods for making permanent specimen of trichinella spiralis encapsulated larvae

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【作者】 都景芳邱娜蔡欢闫钰鑫樊航张澎娄源航王国英

【Author】 DU Jingfang;QIU Na;CAI Huan;YAN Yuxin;FAN Hang;ZHANG Peng;LOU Yuan- hang;WANG Guoying;Medical College,Henan University;Minsheng College,Henan University;

【机构】 河南大学医学院河南大学民生学院

【摘要】 目的比较4种HE染色方法制作旋毛虫肌幼虫囊包标本的效果。方法 HE染色方法1:苏木精染色5min,伊红染色5min,分色;HE染色方法2:苏木精染色15min,伊红染色5min,分色;HE染色方法3:苏木精染色1h,伊红染色30min,分色;HE染色方法4:苏木精染色1h,伊红染色1h,分色。结果 HE染色方法4制作的旋毛虫肌幼虫囊包标本,囊包梭形显著,结构清晰,囊内幼虫特征典型。结论 HE染色方法4制作的标本,染色效果好,易于观察。

【Abstract】 Objective A comparative staining effect of four HE staining methods for specimen of Trichinella spiralis encapsulated larvae.Methods No.1The speciemens were stained with hematoxylin staining solution for 5min and eosin staining solution for 5min,and faded.No.2The speciemens were stained with hematoxylin staining solution for 15 min and eosin staining solution for 5 min,and faded.No.3The speciemens were stained with hematoxylin staining solution for 1hand eosin staining solution for 30 min,and faded.No.4The speciemens were stained with hematoxylin staining solution for 1hand eosin staining solution for 1h,and faded.Results The results showed that with the HE staining method No.4,the specimens showed clear structures and obvious shuttle figure.ConclusionThe encapsulated larvae had a representative character,and easy to be observed.

【关键词】 旋毛虫肌幼虫囊包标本染色
【Key words】 trichina spiralisencapsulated larvaespecimenstaining
【基金】 河南省教育改革资助项目(2014SJGLX137)
  • 【文献出处】 河南大学学报(医学版) ,Journal of Henan University(Medical Science) , 编辑部邮箱 ,2015年03期
  • 【分类号】R383.15
  • 【下载频次】121
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