[Objectives] To examine the expression of the Bombyx mori serine proteinase inhibitor 20 gene(Bm Serpin-20) in Escherichia coli; and more specifically, to analyze its m RNA and protein expression levels in tissues, and its m RNA expression patterns in fat bodies after exogenous microorganism injection. [Methods] The Bm Serpin-20 gene was inserted into the p ET-28 a vector. Recombinant plasmids were transfected into E. coli Transetta(DE3), and expression of the Bm Serpin-20 protein induced with IPTG. The rec...
根据氨基酸序列及蛋白结构不同,蛋白酶抑HP6形成共价复合物阻止了蛋白酶的级联反应,制剂一般被分为4类:丝氨酸蛋白酶抑制剂抑制黑化反应从而发挥免疫效应(Tong et al.,(Serine proteinase inhibitor)、半胱氨酸类蛋白2005)。酶抑制剂、天冬氨酸类蛋白酶抑制剂和金属蛋白对家蚕B
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