This study aims to identify the evolutionary process of the sex determining gene transformer 2 by lots of molecular genetic technologies. The finds will provide basic knowledges for further understanding of the mechanism of sex determination in insects. The full- length c DNA sequence and its intronic seqences of transformer 2 of Bactrocera cucurbitae were isolated by PCR and RACE technique. Meanwhile,the sequence characteristic,phylogeny and molecular evolution of transformer 2 were analyzed by using diffe...