【摘要】 目的探索不同中药提取物对幽门螺杆菌(helicobter pylori,H.pylori)耐药菌株是否具有体外抗菌活性,为中药治疗H.pylori耐药菌株提供依据。方法采用琼脂稀释法分别检测不同中药乙醇提取物对H.pylori标准菌株和临床耐药菌株的最小抑菌浓度(MIC),分别计算其MIC50和MIC90。结果中药提取物大黄、黄连、黄芩、败酱草、延胡索的MIC50和MIC90分别为:32,64,32,64,128,256,>512,>512,>512,>512 mg/L。结论大黄及黄连提取物对H.pylori临床耐药菌株具有较明显的体外抑菌作用;黄芩提取物对H.pylori临床耐药菌株具较弱的体外抑菌作用;败酱草、延胡索提取物对于H.pylori临床耐药菌株无明显体外抑菌作用。更多还原

【Abstract】 Objective To find antibacterial activity of traditional Chinese herbal medicine extracts on antibiotic-resistant Helicobacter pylori( H. pylori) strains in vitro,which could provide the evidence for treating antibiotic-resistant H. pylori strains with Chinese herbal medicines. Methods Agar dilution was used to detect the minimum inhibitory concentration( MIC) of standard strain and clinical drug resistant strain different of traditional Chinese herbal medicine extracts,and MIC50 and MIC90 were calculated.Results The MIC50 and MIC90 of traditional Chinese medicine extracts on H. pylori clinical isolates were as follows respectively: rhubarb: 32 mg / L and 64 mg / L,Rhizoma Coptidis: 32 mg / L and 64 mg /L,radix scutellariae: 128 mg / L and 256 mg / L,herba patriniae > 512 mg / L and > 512 mg / L,rhizoma corydalis > 512 mg / L and > 512 mg / L. Conclusions The results of this study suggest that the extracts of rhubarb and rhizoma coptidis have obvious antibacterial activity,while radix scutellariae has weak antibacterial activity,on H. pylori clinical antibiotic resistant isolates in vitro,herba patriniae and rhizoma corydalis have no significant antibacterial activity.更多还原