【摘要】 目的评估强生VITROS FUSION 5.1全自动干片式生化仪校准品(KIT1:LOT0112;KIT3:LOT0322)两个校准包的基质效应。方法以Olympus AU 5400全自动生化分析仪为参比系统,VITROS FUSION 5.1干化学分析仪为待评估系统,参照CLSI EP14-A2文件对KIT1及KIT3校准包每个校准包3个水平随机排列在20份新鲜的患者标本之中,用待评估系统和参比系统分别对患者标本、KIT1进行血糖(GLU)、尿素(UREA)、肌酐(CR)、尿酸(UA)测定,对KIT3校准包进行天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶(CK)测定。结果 KIT1校准包中GLU、CR相对于参比系统不存在基质效应,其他项目至少有一个水平存在基质效应,KIT3校准包中所有项目至少有一个水平存在基质效应。结论在日常检测中由仪器校准品基质效应造成不同系统间结果的差异是不能忽视的,应尽量减少基质效应带来的影响。通过定期开展不同仪器间比对是减少其影响的手段之一,也是实验室标准化过程中不可或缺的一部分。更多还原

【Abstract】 Objective To evaluate the matrix effect of the calibration packages of calibrator KIT1:LOT 0112 and KIT3:LOT 0322 for the Johnson VITROS FUSION 5.1automatic dry chip analyzer.Methods Using the Olympus AU5400 automatic biochemistry analyzer as the reference system,and the VITROS FUSION 5.1dry type chemical analyzer as the undetermined evaluation system,referring to the NCCLS EP14-A2 files,three levels for each package in the calibration packages of KIT1 and KIT3were randomly arranged in 20 fresh patient samples,GLU,UREA,Cr and UA were detected for the calibrator KIT1 and AST,LDH and CK were detected for the calibrator KIT3 by the reference system and undetermined evaluation system.Results Compared with the reference system,GLU and CR in the KIT1 calibration package had no matrix effects and other items had at least a level with the presence of matrix effects and among all items in KIT3 calibration package at least a level had the presence of matrix effect.Conclusion In daily detection,the difference between the results of different systems caused by the matrix effect of the instrument calibrator can not be ignored.Therefore the effect of matrix effect should be reduced as far as possible.Launching the comparison among different instruments at regular intervals is one of reducing its influence,and also an indispensable part of laboratory standardization.更多还原