【摘要】 目的探讨Ⅳ型胶原(Co-Ⅳ)、层黏连蛋白(LN)及67 k Da层黏蛋白受体(67LR)在肺癌中表达及相关性,旨在发现潜在反映肺癌恶性的生物学行为及预后指标。方法采用免疫组织化学链霉菌生物素蛋白-过氧化酶法(SP),分别检测Co-Ⅳ、LN、67LR 3种抗原成分在不同组织类型、不同分化程度的84例肺癌原发灶及52例转移灶中的表达分布,并进行比较和相关分析。结果 Co-Ⅳ、LN的表达在鳞癌和腺癌的高、低分化原发灶各组间比较,差异均有统计学意义(P<0.05);高分化癌高表达,低分化癌低表达;在各病理类型原发灶与转移灶阳性表达上,除鳞癌组外,其他各组间比较,差异均有统计学意义(P<0.05),原发灶表达高于转移灶。67LR在鳞癌和腺癌高、低分化组间表达,差异均有统计学意义(P<0.05);高分化癌中低表达,低分化癌中高表达。Co-Ⅳ、LN、67LR在鳞癌和腺癌高、低分化组间表达具有相关性(P<0.01)。Co-Ⅳ与67LR、LN在病理组织类型上各组间表达,差异无统计学意义(P>0.05);但在有无淋巴转移组间和不同生存期组间,差异均有统计学意义(P<0.05)。Co-Ⅳ、LN在有淋巴转移组和生存期≥4年组高表达,而67LR低表达。结论对Co-Ⅳ、LN、67LR表达的综合检测能为判断肺癌的恶性生物学行为提供更多的信息,可望成为揭示肺癌转移潜能和评测预后的指标。更多还原

【Abstract】 Objective To investigate the relationship and difference between the expressions of three protein antigens,LN,67 LR and Collagen Ⅳ( Co-Ⅳ),in order to find the factors which can forecast malignant biological behaviors of lung cancers and predict the prognosis of the patients for guiding clinical therapeutics. Methods The expression of LN,67 LR and Co-Ⅳ were examined by immunohistochemical SP method( Streptavidin-Peroxidase,SP) in 84 cases of lung cancer and their 52 lymphatic metastasis,in order to analyze the different expression and distribution of three antigens among different tissue types and differentiation of lung cancers.Also,different expression of three antigens was compared. Results The positⅣ e expression of Co-Ⅳ and LN protein were significant different( P < 0. 05) in well and poor differentiation of squamous cell carcinoma( SQCC),and the same in adenocarcinoma( ADC),P < 0. 05. The well differentiation groups express higher than the poor ones in BM around SQCC and ADC. The other groups between primary tumors and metastasis also have significant differences( P < 0. 05) at every pathological type groups except SQCC groups. In the poor differentiation SQCC and ADC,the positive expression of 67 LR was higher than that in the well( P < 0. 05). The correlations in three protein antigens expressions could be found in well and poor differentiation of SQCC and ADC,while,the significant differences were not found among different pathological type groups( P > 0. 05). The other significant differences were found between the metastasis and no metastasis groups,between subsistent time more than 4 years and less than 4 years groups.Conclusion Synthetical study on expression of Co-Ⅳ,LN and 67 LR can provide more information about malignant behavior of lung cancers. It may be the factors which can forecast metastasis ability and prognosis of the patients with lung cancers.更多还原