【摘要】 以沉水樟当年生半木质化枝条为材料,通过试验设计得到沉水樟最适诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.2 mg/L(p H=6.0),最佳继代增殖培养基和生根培养基分别为1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+Na2S2O3150mg/L(p H=5.5)和1/2MS+IBA 1.0 mg/L+NAA 0.05 mg/L,增殖系数达到2.5,有效生根率达到96%,且在增殖培养基中添加一定浓度的Na2S2O3,解决了新抽芽顶梢或叶枯顶或掉叶难题,为沉水樟快繁提供了一种有效方法。更多还原

【Abstract】 One-year half-lignified shoots from Cinnamomum micranthum trees were used to study the optimum media for tissue culture. The results showed that optimum induction medium for C. micranthum is MS+6-BA 0.5 mg/L+NAA 0.2 mg/L(p H=6.0). The best proliferation medium is 1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+ Na2S2O3150 mg/L(p H=5.5), and its multiplication coefficient can reach 2.5. Rooting rate is above 96% in the optimum rooting medium that is 1/2MS+IBA1.0 mg/L +NAA0.05 mg/L. Moreover, proliferation medium containing certain concentration Na2S2O3 can efficiently resolve the difficult problem that the shoot top withered up or leaves fallen during the process of the proliferation culture, and provide an effective method for rapid propagation of C. micranthum.更多还原