【摘要】 目的探讨HCV-cAg和HCV-Ab两个指标联合检测对HCV感染的临床价值。方法对门诊和住院患者血液标本进行HCV-cAg、HCV-Ab及HCV-RNA联合检测,采用实时荧光定量PCR法检测HCV-RNA,采用酶联免疫吸附法(ELISA)检测试剂盒检测HCV-Ab和HCV-cAg。结果 HCV-Ab检测的灵敏度和特异度分别为90.91%和99.17%;HCV-cAg检测的灵敏度和特异度分别为70.25%和100%,两者的特异度相近,但是HCV-Ab检测的灵敏度比HCV-cAg的要高。两者联合检测时灵敏度和特异度分别为99.17%和99.17%,灵敏度明显增加。此外,HCV-Ab和HCV-cAg两者之间的检测结果无一致性。结论 HCV-Ab和HCV-cAg检测相互间无法替代,将抗-HCV和HCV-cAg两种指标结合起来检测,对临床上提高HCV感染的筛查和诊断具有重要的价值。更多还原

【Abstract】 Objective To explore the clinical value of combination of HCV-cAg and anti-HCV detection in HCV infection.Methods The serum samples from outpatients and inpatients were detected for HCV-cAg,anti-HCV and HCV-RNA. The quantitative real-time PCR was applied to detect HCV-RNA,and enzyme-linked immunosorbent assay(ELISA) kits were utilized to test anti-HCV and HCV-cAg). Result The sensitivity and specificity of anti-HCV detection were 90.91% and 99.17% respectively,and that of HCV-cAg were 70.25% and 100% respectively. Notably,the sensitivity(99.17%) and specificity(99.17%) increased significantly in case of combinational detection method. In addition,no consistency between the results of anti-HCV and HCV-cAg was detected. Conclusion Combination detection of anti-HCV and HCV-cAg was recommended because of its remarkable advantage in screening and diagnosis of HIV infection.更多还原