【摘要】 可选择性多聚腺苷酸化(alternative polyadenylation,APA)是一种普遍存在于真核生物中的基因调控机制。APA事件的发生能使一个基因产生多种mRNA转录本,从而增加转录本的复杂度。选择不同的多聚腺苷酸化位点可以使不同的转录本具有不同的编码序列,或具有不同长度的3’端非翻译区(3’untranslated region,3’UTR)。不同长度的3’UTR可引起RNA结合蛋白或微小RNA(miRNA)的结合位点发生变化,进而影响mRNA的稳定性、定位和翻译效率。近年来,APA的研究涉及APA的形成过程、调控APA的机制以及APA对生物学过程和疾病的影响。该文对APA的上述研究进展进行了综述。更多还原

【Abstract】 Alternative polyadenylation( APA) is a widespread phenomenon and an important layer of gene regulation.APA contributes to the complexity of the transcriptome by allowing a single gene to encode multiple mRNA isoforms that differ either in their coding sequence or in their 3’ untranslated regions( 3’UTR). The length of the 3’UTR can affect the stability,localization and efficiency of the messenger RNAs( mRNAs) by altering binding sites of RNA binding proteins or microRNAs( miRNAs). The polyadenylation process,mechanisms governing APA and biological consequences resulting from APA are only starting to be deciphered. Here,we review the research progress in APA.更多还原