【摘要】 目的对徐州市HIV-1/2抗体筛查阳性标本进行复检和确证实验。方法按《全国艾滋病检测技术规范(2009年修订版)》要求,对2012-2013年徐州市辖区内的艾滋病筛查实验室送检的HIV-1/2抗体筛查阳性标本进行复检实验(ELISA和胶体硒),复检阳性标本进行确证实验(免疫印迹法),对确证结果为不确定者进行随访复查。结果 678份筛查阳性标本中复检HIV-1抗体阳性434份,复检阳性率64.01%;确证实验阳性400份,占92.17%;不确定27份,占6.22%;阴性7份,占1.61%。400份确证阳性的标本中,有397份标本酶联免疫法筛查实验中的S/CO值>6,占99.25%;34份确证阴性和不确定样本中,21份S/CO值<6,占61.76%。确证阳性标本中出现≥7个条带的375份,占93.75%。27例不确定者随访到16例,其中14例结果阳转,2例转阴。结论筛查实验存在一定的假阳性,S/CO值>6的标本确证阳性的几率较高;对同时出现gp160和p24的不确定样本应予以重视。更多还原

【Abstract】 Objective To re-examine and confirm samples which were positive in primary HIV-1/2 antibody screening tests in Xuzhou.Methods According to National Guideline for Detection of HIV/AIDS(2009),positive samples in primary HIV-1/2 antibody screening tests from all HIV primary screening laboratories in Xuzhou from 2012 to 2013 were subject to reexaminations(by ELISA and Colloidal Selenium assay),positive samples were confirmed by Western Blot analysis.Cases with uncertain results in the confirmatory tests were subjected to followed up management.Results Among the 678 HIV positive samples in primary screening,434 were positive in re-examination test,resulting positive rate of 64.01%;of which 400 samples were confirmed to be HIV antibody positive,accounting for 92.17%;27 samples’ results were uncertain,accounting for6.22%;7 samples were negative,accounting for 1.61%.Among 400 confirmed samples,397 samples resulted S/CO>6 in ELISA,accounting for 99.25%;for 34 samples with negative and uncertain results,21 resulted S/CO<6,accounting for 61.76%.375 of 400 confirmed positive samples had more than 7stripes,accounting for 93.75%.Among 27 uncertain cases,16 cases were subjected to followed up management,in which 14 cases converted positive and 2cases converted negative.Conclusions There are some false positive in primary screening tests,specimens with S/CO values> 6had higher probability to be confirmed as positive samples.More attention should be paid to uncertain samples with simultaneous occurrence of gp160 and p24.更多还原