【摘要】 目的对2014年江苏省一起疑似人感染猪链球菌病的病原菌进行实验室快速检测并分析其分子特征。方法采用实时荧光PCR方法对病人标本中提取的DNA进行猪链球菌血清2型特异基因检测,运用PCR方法进行猪链球菌毒力基因鉴定及多位点序列分析。结果病人标本中检测到猪链球菌种特异基因(16srRNA)和血清2型荚膜多糖编码基因(cps-2J),5种毒力基因(mrp,sly,ef,gapdh,fbps)全部阳性,多位点序列分析结果为ST1型。结论该病例的病原菌为携带5种毒力基因的ST1型猪链球菌血清2型菌株,该型别在江苏省尚属首次报道。更多还原

【Abstract】 Objective To apply rapid detection and molecular characteristics of pathogen in 1suspected-Streptococcus suis infection case in Jiangsu Province,2014.Methods DNA was extracted from patient’s specimens.PCR was used to detect specific gene of Streptococcus suis serotype 2and identification of virulence genes followed by multi-locus sequence typing analysis.Results Streptococcus suis specific gene(16srRNA)and serotype 2specific gene encoding Capsular polysaccharide(cps-2J),together with 5virulence genes(mrp,sly ef,gapdh and fbps)were all positive in PCR reactions.Multi-locus sequence typing analysis showed pathogen was type 1 Streptococcus suis(ST1).Conculsion It was a case of human infection with type 1Streptococcus suis of serotype 2carrying 5virulence genes,which was first reported in Jiangsu Province.更多还原