【摘要】 采用结晶紫染色检测结核分枝杆菌生物被膜的形成能力,实时荧光定量PCR(qRT-PCR)检测生物被膜态的不同结核分枝杆菌Rv3519表达水平,分析Rv3519基因表达与结核分枝杆菌生物被膜形成能力的关系。结果表明:临床分离株产膜能力较H37Rv明显偏低,差异显著(P<0.01);临床分离株之间生物被膜产量差异不显著(P>0.05);生物被膜态的分离株较标准株Rv3519基因表达量偏低,差异显著(P<0.01)。说明结核分枝杆菌生物被膜形成能力与Rv3519基因表达水平相关,Rv3519基因可以作为结核分枝杆菌生物被膜形成能力的潜在分子诊断标志。更多还原

【Abstract】 Biofilm formation ability of Mycobacterium Tuberculosis(M.tuberculosis)was measured by crystal violet staining.Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the levels of expression of Rv3519 gene in the biofilms of different M.tuberculosis stains.The relationship between Rv3519 gene expression and the level of their biofilm formation ability was explored.Results showed that the ability of biofilm formation of H37 Rv was stronger than those of clinical isolates,which was significantly different(P<0.01);there was no significant difference among the biofilm formation ability of clinical isolates(P>0.05);the expression level of Rv3519 of H37Rv was significantly higher than that of the clinical isolates(P<0.01).In short,there was a correlation between the biofilm-forming ability and Rv3519 gene expression level of M.tuberculosis strains,and Rv3519 gene can be regarded as a potential molecular diagnostic marker for biofilm-forming ability of M.tuberculosis.更多还原