upp基因可用来作为反向筛选标记基因。利用p ORI温度敏感型双质粒系统敲除干酪乳杆菌Lactobacillus casei 393的upp基因,构建得到重组菌Lactobacillus casei 393-△upp。重组菌可作为反向筛选标记基因upp使用的宿主菌。
【英文摘要】
Upp gene exists widely in prokaryotes and some primary eukaryotes cells. UPRT( Uracil Phosphoribosyl transferase),encoded by upp gene,can convert 5-fluorouracil to 5-fluoro-d UMP,which inhibits thymidylate synthase,causing cell death. So the upp gene can be used as a counterselectable marker. In this study,we construst Lactobacillus casei 393-△upp mutant by p ORI temperature-sensitive dual plasmid system and the upp counterselectable marker can be used in this upp deleted strain.
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